Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Postfach 2148, D-4630, Bochum, Federal Republic of Germany.
Planta. 1976 Jan;131(1):33-40. doi: 10.1007/BF00387342.
A procedure is described which permits the estimation of the relative activity of phenylalanine ammonia-lyase (E.C. 4.3.1.5.) in intact plant cells, exemplified by buckwheat hypocotyls. Hypocotyl segments are incubated at pH 5.5 with L-[3-(3)H]phenylalanine. N(3)HH2, which is liberated from phenylalanine by the action of phenylalanine ammonia-lyase, equilibrates with tissue water to yield (3)HOH, which is recovered by sublimation. Participation of phenylalanine transaminase in the reactions leading to (3)HOH formation is excluded, and it is conclusively shown that (3)HOH is formed intracellularly and not by enzymatic activity leaking out of wounded tissue.
描述了一种方法，该方法允许估计苯丙氨酸解氨酶（E.C.4.3.1.5.）在完整植物细胞中的相对活性，以荞麦下胚轴为例。在下胚轴段在 pH 5.5 下用 L-[3-(3)H]苯丙氨酸孵育。由苯丙氨酸解氨酶作用从苯丙氨酸中释放出来的 N(3)HH2 与组织水达到平衡，生成（3）HOH，（3）HOH 通过升华回收。排除了苯丙氨酸转氨酶参与导致（3）HOH 形成的反应，并且可以明确地证明（3）HOH 是在细胞内形成的，而不是由受伤组织漏出的酶活性形成的。
