Lehrstuhl für Pflanzenphysiologie, Ruhr-University Bochum, Postfach 2148, D-4630, Bochum, Federal Republic of Germany.
Planta. 1976 Jan;131(1):41-5. doi: 10.1007/BF00387343.
Fluctuations in the activity of phenylalanine ammonia-lyase (PAL) in tissues of buckwheat seedlings were monitored by an intact cell assay and compared with results obtained with the conventional in vitro assay. Good correlation between measurements with the different assays was found for PAL activity changes during 1) growth of seedlings in the dark, 2) illumination of seedlings, and 3) incubation of hypocotyl segments. When leaf disks of buckwheat, strawberry, or sunflower were allowed to float on either water or a sugar solution, PAL activities determined with the intact cell assay increased irrespective of the presence of sugar, while PAL activities determined in cell homogenates increased only in disks floating on a sugar solution. Possible explanations for these discrepancies are discussed.
通过完整细胞测定法监测荞麦幼苗组织中苯丙氨酸解氨酶（PAL）的活性变化，并将其与传统的体外测定法的结果进行比较。在 1）幼苗在黑暗中生长，2）幼苗光照，和 3）下胚轴片段培养期间，不同测定法之间的 PAL 活性变化的测量结果之间存在良好的相关性。当荞麦、草莓或向日葵的叶圆片被允许漂浮在水或糖溶液上时，用完整细胞测定法测定的 PAL 活性无论是否存在糖都会增加，而在细胞匀浆中测定的 PAL 活性仅在漂浮在糖溶液上的圆片上增加。讨论了这些差异的可能解释。
