Miège M N, Mascherpa J M, Royer-Spierer A, Grange A, Miège J
Laboratoire de Chimie Taxonomique, Institut de Botanique Systématique de l'Université de Genève, 1. chemin de l'Impératrice, CH-1292, Chambesy, Switzerland.
Planta. 1976 Jan;131(1):81-6. doi: 10.1007/BF00387349.
Cotyledonary cells are submitted to fractionation by isopycnic centrifugation. Small intact protein bodies are collected in the densest zones (d=1.205-1.237 g/cm(3)). Fragments of larger bodies are gathered in zones of lower density (d=1.205 g/cm(3)). Small dense bodies are largely sedimentable after dilution, whereas fragments of the large bodies dissociate into a dense sedimentable clot and into floating elements which contain most of the globulins and all of the albumins. Among the dissociated floating components are the BAPA-active endopeptidases and the trypsin inhibitors (BAPA=α-N-benzoyl-DL-arginine-p-nitroanilide). A caseolytic activity remains with the dense mass. The localisation of the albumins, globulins, proteases and trypsin inhibitors is discussed. Relations between solubility, structure and function are considered.
子叶细胞通过等密度离心进行分级分离。小的完整蛋白体收集在密度最大的区域（d = 1.205 - 1.237 g/cm³）。较大蛋白体的片段聚集在密度较低的区域（d = 1.205 g/cm³）。稀释后，小的致密体大部分可沉淀，而大蛋白体的片段则解离成致密的可沉淀凝块和漂浮成分，其中包含大部分球蛋白和所有白蛋白。解离的漂浮成分中有BAPA活性内肽酶和胰蛋白酶抑制剂（BAPA = α - N - 苯甲酰 - DL - 精氨酸 - 对硝基苯胺）。致密团块仍具有酪蛋白分解活性。讨论了白蛋白、球蛋白、蛋白酶和胰蛋白酶抑制剂的定位。考虑了溶解度、结构和功能之间的关系。
