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World J Microbiol Biotechnol. 1991 Jan;7(1):53-60. doi: 10.1007/BF02310920.
The nutritional requirements and growth characteristics of a biosurfactant-producingRhodococcus bacterium isolated from Kuwaiti soil were determined. Maximum cell yields (6.6 g/l) and biosurfactant production were achieved with a medium containing 2% (v/v)n-paraffin as a carbon and energy source, 0.2% lactose broth, optimal concentrations of nitrogen (nitrate), phosphorus, iron, magnesium and sodium sources, and minimal concentrations of potassium and trace element sources. The optimal pH was 6.8 for surfactant production and optimal temperature was 37°C. The biosurfactant produced after 16 to 33 h growth in a 7 I fermenter decreased both surface tension and interfacial tension of culture broth to below 27 and 1.8 mN/m, respectively, and was effective at critical micelle dilutions of 10(-3). Data on biosurfactant biosynthesis suggest that the product is produced as a primary metabolite and, therefore, could be produced effectively under continuous fermentation conditions.
从科威特土壤中分离出的产生生物表面活性剂的罗霍氏菌的营养需求和生长特性已确定。在含有 2%（v/v）正构石蜡作为碳源和能源、0.2%乳糖肉汤、最佳浓度的氮（硝酸盐）、磷、铁、镁和钠源以及最小浓度的钾和微量元素源的培养基中，可实现最大细胞产量（6.6 g/l）和生物表面活性剂的生产。表面活性剂生产的最佳 pH 值为 6.8，最佳温度为 37°C。在 7 I 发酵罐中生长 16 至 33 小时后产生的生物表面活性剂将培养液的表面张力和界面张力分别降低至低于 27 和 1.8 mN/m，并且在临界胶束稀释度为 10（-3）时有效。生物表面活性剂生物合成的数据表明，该产物是作为初级代谢产物产生的，因此可以在连续发酵条件下有效地生产。
