Cooper D G, Zajic J E, Gerson D F
Appl Environ Microbiol. 1979 Jan;37(1):4-10. doi: 10.1128/aem.37.1.4-10.1979.
Corynebacterium lepus was grown in 20-liter batch fermentations with kerosene as the sole carbon source. Critical micelle concentration measurements indicated the production of appreciable quantities of biosurfactants. This surface activity of the culture medium was due to lipids, which were extracted and identified. Samples of C. lepus whole broth were taken during a fermentation and monitored for surface tension, amount of surfactant present, and lipid content. The changes in the surfactant measured correlated with concentration changes of several surface-active lipids. An early dramatic increase in surfactant concentration was attributed to the production of a mixture of corynomycolic acids (beta-hydroxy alpha-branched fatty acids). Surface activity at the end of the fermentation was due to a lipopeptide containing corynomycolic acids plus small amounts of several phospholipids and neutral lipids which were identified by thin-layer chromatography.
以煤油作为唯一碳源，在20升的分批发酵罐中培养兔棒状杆菌。临界胶束浓度测量表明产生了相当数量的生物表面活性剂。培养基的这种表面活性归因于脂质，对脂质进行了提取和鉴定。在发酵过程中采集兔棒状杆菌全培养液样品，并监测其表面张力、表面活性剂含量和脂质含量。所测表面活性剂的变化与几种表面活性脂质的浓度变化相关。表面活性剂浓度早期的急剧增加归因于棒状菌酸（β-羟基α-支链脂肪酸）混合物的产生。发酵结束时的表面活性归因于一种含有棒状菌酸的脂肽，以及通过薄层色谱法鉴定出的少量几种磷脂和中性脂质。
