Department of Botany and Microbiology, University College of Wales, SY23 3DA, Aberystwyth, UK.
Planta. 1976 Jan;132(1):9-12. doi: 10.1007/BF00390324.
Mature antheridia from agar cultures of prothalli of the bracken fern were fixed for short periods of time in buffered 1% glutaraldehyde. Cryosections were obtained after rapid freezing in glycerol. In oblique cryo-sections through flagella rows of small globular subunits were observed making up each axoneme, the approximate size of each globule was 4.5 nm. Other superficial material was also found in the same sections. In transverse sections through the layer of microtubules which surround the helical nucleus, subunits of the order of 4.5 nm were observed.
从蕨类植物原叶体的琼脂培养物中成熟的精囊，在缓冲的 1%戊二醛中固定很短的时间。快速冷冻在甘油中后获得冷冻切片。在通过鞭毛行的斜冷冻切片中观察到每个轴丝由小的球形亚基组成，每个小球的大致尺寸为 4.5nm。在同一切片中还发现了其他表面物质。在穿过围绕螺旋核的微管层的横切面上，观察到了大约 4.5nm 的亚基。
