Department of Botany, University of Aberdeen, AB9 2UD, Aberdeen, U.K..
Planta. 1976 Jan;132(2):97-102. doi: 10.1007/BF00388889.
Proteins in sieve tube exudate from Ricinus communis L., Acer pseudoplatanus L., Aesculus hippocastanum L., Cucumis melo L., and two cultivars each of Cucumis sativus L., Cucurbita pepo L. and Cucurbita maxima Duchesne were fractionated and compared using polyacrylamide gel electrophoresis. Striking differences in major exudate proteins were displayed among the genera and species examined. Even cultivars within a single species, although showing general similarities, differed in some prominent proteins. Estimated molecular weights of the major exudate proteins from each plant are presented. The effects of reducing and chaotropic agents on the aggregation and subunit composition of exudate proteins from Cucumis sativus have been investigated. The problems involved in relating structure, function and biochemistry of P-protein are discussed.
蓖麻、槭树、七叶树、甜瓜和 2 个不同品种的黄瓜、南瓜的筛管分泌蛋白经聚丙烯酰胺凝胶电泳进行了分离和比较。研究的属和种之间的主要分泌蛋白存在显著差异。即使是同一物种的不同品种，尽管表现出一般的相似性，但在一些突出的蛋白质上也存在差异。每个植物的主要分泌蛋白的估计分子量也已给出。还研究了还原和张性试剂对黄瓜分泌蛋白聚集和亚基组成的影响。讨论了与 P 蛋白的结构、功能和生物化学相关的问题。
