一种来自西葫芦（南瓜属西葫芦）果实渗出物的凝集素，它对β-1,4-连接的N-乙酰葡糖胺寡糖具有特异性。
A lectin from the exudate of the fruit of the vegetable marrow (Cucurbita pepo) that has a specificity for beta-1,4-linked N-acetylglucosamine oligosaccharides.
作者信息
Allen A K
出版信息
Biochem J. 1979 Oct 1;183(1):133-7. doi: 10.1042/bj1830133.
Abstract
Lectins are present in the exudate (presumably from the phloem) of the fruits of three species of the Cucurbitaceae, namely vegetable marrow (Cucurbita pepo), melon (Cucumis melo) and cucumber (Cucumis sativus). They are all strongly inhibited in their activities by chitin oligosaccharides, but only weakly by N-acetylglucosamine. Glycopeptides from soya-bean agglutinin and fetuin are also strong inhibitors of Cucurbita pepo lectin, indicating that it interacts with internal N-acetylglucosamine residues. The lectin from Cucurbita pepo fruit was purified by affinity chromatography by using chitin oligosaccharides covalently attached to Sepharose. The lectin is not a glycoprotein, and it consists of a single polypeptide chain of about 20,000 mol.wt. It is a major protein (18% of the total) of the phloem exudate and it is postulated that it may have an anti-parasitic function.
摘要
凝集素存在于葫芦科三种植物果实的渗出液（可能来自韧皮部）中，这三种植物分别是西葫芦（南瓜属西葫芦）、甜瓜（甜瓜属甜瓜）和黄瓜（黄瓜属黄瓜）。它们的活性都受到几丁质寡糖的强烈抑制，但仅受到N - 乙酰葡糖胺的微弱抑制。来自大豆凝集素和胎球蛋白的糖肽也是西葫芦凝集素的强抑制剂，这表明它与内部的N - 乙酰葡糖胺残基相互作用。通过使用共价连接到琼脂糖上的几丁质寡糖，通过亲和色谱法纯化了西葫芦果实中的凝集素。该凝集素不是糖蛋白，它由一条分子量约为20,000的单多肽链组成。它是韧皮部渗出液中的一种主要蛋白质（占总量的18%），据推测它可能具有抗寄生虫功能。
相似文献
1
A lectin from the exudate of the fruit of the vegetable marrow (Cucurbita pepo) that has a specificity for beta-1,4-linked N-acetylglucosamine oligosaccharides.一种来自西葫芦（南瓜属西葫芦）果实渗出物的凝集素，它对β-1,4-连接的N-乙酰葡糖胺寡糖具有特异性。
Biochem J. 1979 Oct 1;183(1):133-7. doi: 10.1042/bj1830133.
2
Some properties of the lectin from Datura stramonium (thorn-apple) and the nature of its glycoprotein linkages.曼陀罗凝集素的一些特性及其糖蛋白连接的性质。
Biochem J. 1981 Aug 1;197(2):345-53. doi: 10.1042/bj1970345.
3
4
The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein.来自马铃薯块茎的凝集素（一种含羟脯氨酸的糖蛋白）的纯化及性质
Biochem J. 1973 Oct;135(2):307-14. doi: 10.1042/bj1350307.
5
Purification of the glycoprotein lectin from the broad bean (Vicia faba) and a comparison of its properties with lectins of similar specificity.从蚕豆（Vicia faba）中纯化糖蛋白凝集素及其与具有相似特异性的凝集素的性质比较。
Biochem J. 1976 Apr 1;155(1):127-35. doi: 10.1042/bj1550127.
6
Purification, physico-chemical characterization and thermodynamics of chitooligosaccharide binding to cucumber (Cucumis sativus) phloem lectin.壳寡糖与黄瓜（Cucumis sativus）韧皮部 lectin 结合的纯化、理化特性表征及热力学。
Int J Biol Macromol. 2017 Feb;95:910-919. doi: 10.1016/j.ijbiomac.2016.10.078. Epub 2016 Oct 25.
7
Interaction of asparagine-linked oligosaccharides with an immobilized rice (Oryza sativa) lectin column.天冬酰胺连接的寡糖与固定化水稻（Oryza sativa）凝集素柱的相互作用。
Biochem J. 1988 Feb 15;250(1):117-24. doi: 10.1042/bj2500117.
8
Protein inhibitors of trypsin from the seeds of Cucurbitaceae plants.葫芦科植物种子中胰蛋白酶的蛋白质抑制剂。
Acta Biochim Pol. 1987;34(4):395-406.
9
Isolation and partial characterization of an N-acetylgalactosamine-specific lectin from winter-aconite (Eranthis hyemalis) root tubers.从菟葵（Eranthis hyemalis）块根中分离并部分鉴定一种N-乙酰半乳糖胺特异性凝集素。
Biochem J. 1985 May 1;227(3):949-55. doi: 10.1042/bj2270949.
10
Rapid affinity-purification and physicochemical characterization of pumpkin (Cucurbita maxima) phloem exudate lectin.南瓜（Cucurbita maxima）韧皮部泌液凝集素的快速亲和纯化及理化特性分析。
Biosci Rep. 2010 Apr 21;30(5):341-9. doi: 10.1042/BSR20090117.
引用本文的文献
1
Exploration of structural space of Cus17 lectin with glycans using molecular docking and simulation studies.利用分子对接和模拟研究探索Cus17凝集素与聚糖的结构空间。
Comput Struct Biotechnol J. 2025 Aug 2;27:3319-3327. doi: 10.1016/j.csbj.2025.07.028. eCollection 2025.
2
PP2 gene family in Phyllostachys edulis: identification, characterization, and expression profiles.雷竹 PP2 基因家族的鉴定、特征分析与表达谱
BMC Genomics. 2024 Nov 13;25(1):1081. doi: 10.1186/s12864-024-11007-5.
3
The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode.Nictaba的晶体结构揭示了其碳水化合物结合特性和一种新的凝集素二聚化模式。
Glycobiology. 2024 Dec 10;34(12). doi: 10.1093/glycob/cwae087.
4
Carbohydrate-Binding Properties and Antimicrobial and Anticancer Potential of a New Lectin from the Phloem Sap of .从. 的韧皮部汁液中提取的一种新凝集素的碳水化合物结合特性以及抗微生物和抗癌潜力
Molecules. 2024 May 27;29(11):2531. doi: 10.3390/molecules29112531.
5
Elucidating the glycan-binding specificity and structure of agglutinin, a new R-type lectin.解析一种新型R型凝集素——凝集素的聚糖结合特异性和结构。
Beilstein J Org Chem. 2024 Feb 19;20:306-320. doi: 10.3762/bjoc.20.31. eCollection 2024.
6
Facile Labeling of Sieve Element Phloem-Protein Bodies Using the Reciprocal Oligosaccharide Probe OGA .使用相互寡糖探针OGA对筛管分子韧皮部蛋白体进行简便标记
Front Plant Sci. 2022 Feb 10;13:809923. doi: 10.3389/fpls.2022.809923. eCollection 2022.
7
Calcium-binding protein in sieve tube exudate.筛管分泌液中的钙结合蛋白。
Planta. 1981 May;151(6):531-4. doi: 10.1007/BF00387430.
8
Chemical and immunological similarities between the phloem proteins of three genera of the Cucurbitaceae.葫芦科三个属韧皮蛋白的化学和免疫学相似性。
Planta. 1983 Jun;158(2):119-27. doi: 10.1007/BF00397704.
9
A lectin from Bryonia dioica root stocks.野葛根凝集素。
Planta. 1984 Mar;160(3):222-8. doi: 10.1007/BF00402858.
10
Isolation and partial characterization of a lectin from ground elder (Aegopodium podagraria) rhizomes.从土大黄（Aegopodium podagraria）根茎中分离和部分表征一种凝集素。
Planta. 1985 May;164(1):75-82. doi: 10.1007/BF00391028.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Further observations on the inhibition of blood-group specific serological reactions by simple sugars of known structure.关于已知结构的单糖对血型特异性血清学反应抑制作用的进一步观察
Vox Sang. 1962;7:129-50. doi: 10.1111/j.1423-0410.1962.tb03238.x.
3
The oxidation of ribonuclease with performic acid.用过甲酸氧化核糖核酸酶。
J Biol Chem. 1956 Apr;219(2):611-21.
4
Protein-carbohydrate interaction. VII. Physical and chemical studies on concanavalin A, the hemagglutinin of the jack bean.蛋白质-碳水化合物相互作用。VII. 刀豆球蛋白A（刀豆的血凝素）的物理和化学研究
Arch Biochem Biophys. 1968 Mar 20;124(1):218-29. doi: 10.1016/0003-9861(68)90322-6.
5
Studies on phytohemagglutinins. 3. Isolation and characterization of hemagglutinins from the pea (Pisum sativum L.).植物血凝素研究。3. 豌豆（Pisum sativum L.）血凝素的分离与特性分析。
Biochim Biophys Acta. 1970 Nov 17;221(2):272-81. doi: 10.1016/0005-2795(70)90267-9.
6
Specific inhibition by N-acetyl-D-galactosamine of the interaction between soybean agglutinin and animal cell surfaces.N-乙酰-D-半乳糖胺对大豆凝集素与动物细胞表面相互作用的特异性抑制作用。
Biochim Biophys Acta. 1970 Sep 15;211(3):582-5. doi: 10.1016/0005-2736(70)90265-8.
7
An assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials.对甲醇解及用于含碳水化合物材料分析的其他因素的评估。
Biochem J. 1971 Dec;125(4):1009-18. doi: 10.1042/bj1251009.
8
The purification and some properties of a protein causing gelling in phloem sieve tube exudate from Cucurbita pepo.西葫芦韧皮部筛管渗出液中一种引起凝胶化的蛋白质的纯化及其某些性质
Biochim Biophys Acta. 1972 Feb 29;257(2):433-44. doi: 10.1016/0005-2795(72)90296-6.
9
The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein.来自马铃薯块茎的凝集素（一种含羟脯氨酸的糖蛋白）的纯化及性质
Biochem J. 1973 Oct;135(2):307-14. doi: 10.1042/bj1350307.
10
The purification, composition and specificity of wheat-germ agglutinin.小麦胚芽凝集素的纯化、组成及特异性
Biochem J. 1973 Jan;131(1):155-62. doi: 10.1042/bj1310155.
Zotero 插件