一种提高免疫原性病毒产量用于疫苗生产的新方法。
A novel method for increased yield of immunocompetent virus for vaccine production.
机构信息
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出版信息
World J Microbiol Biotechnol. 1991 Sep;7(5):567-70. doi: 10.1007/BF00368362.
PMID:24425199
Abstract
Cultured human cells exposed to the pesticide emulsifier Atlox, 6 to 8 h prior to Infection with influenza A virus, increased virus production approximately 10-fold. Antibodies against the 'enhanced virus' neutralized plaque formation and reacted equally well with non-enhanced virus in serological tests (haemagglutination-inhibition and radioimmunoassays). The procedure has great potential in cutting costs of production for some virus vaccines.
摘要
在感染甲型流感病毒前，将人类细胞在农药乳化剂 Atlox 中培养 6 到 8 小时，可使病毒产量增加约 10 倍。针对“增强型病毒”的抗体可中和噬菌斑形成，并在血清学检测（血凝抑制和放射免疫测定）中与非增强型病毒同样反应良好。该方法在降低某些病毒疫苗的生产成本方面具有巨大潜力。
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