Qureshi A A, Trent D W
Infect Immun. 1973 Feb;7(2):242-8. doi: 10.1128/iai.7.2.242-248.1973.
Saint Louis encephalitis virus-infected cells were solubilized with Brij-58 nonionic detergent, and the clarified supernatant fluid was analyzed by diethyl-aminoethyl-cellulose chromatography. Three serologically distinguishable antigens were identified in viral protein peaks which eluted from diethylaminoethyl-cellulose at 0.05, 0.075, 0.125 and 0.2 m KCl. Antigen I was found in all column peaks, whereas antigen II was found only in the 0.05 m KCl eluate and antigen III was found only in the 0.2 m eluate. Immunodiffusion studies revealed that antigens I and II share at least one antigenic determinant and that antigen III is immunologically distinct from either antigen I or II. Polyacrylamide gel electrophoresis of (3)H-leucine-labeled concentrated column peaks revealed that antigens I and II contain the major viral structural envelope and core proteins, respectively. Antigen III contains the largest virus-induced nonstructural polypeptide observed by polyacrylamide gel electrophoresis of (3)H-leucine-labeled Saint Louis encephalitis virus-infected cells.
用Brij - 58非离子去污剂溶解感染圣路易斯脑炎病毒的细胞，然后通过二乙氨基乙基纤维素色谱法分析澄清的上清液。在从二乙氨基乙基纤维素以0.05、0.075、0.125和0.2 m KCl洗脱的病毒蛋白峰中鉴定出三种血清学上可区分的抗原。抗原I存在于所有柱峰中，而抗原II仅存在于0.05 m KCl洗脱液中，抗原III仅存在于0.2 m洗脱液中。免疫扩散研究表明，抗原I和II至少共享一个抗原决定簇，并且抗原III在免疫上与抗原I或II不同。对用³H - 亮氨酸标记的浓缩柱峰进行聚丙烯酰胺凝胶电泳显示，抗原I和II分别包含主要的病毒结构包膜蛋白和核心蛋白。抗原III包含在用³H - 亮氨酸标记的感染圣路易斯脑炎病毒的细胞进行聚丙烯酰胺凝胶电泳时观察到的最大的病毒诱导非结构多肽。
