Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka 564-8680 Japan.
Division of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Kitami, Hokkaido 090-8507 Japan.
J Food Sci Technol. 2013 Apr;50(2):266-74. doi: 10.1007/s13197-011-0343-y. Epub 2011 Mar 30.
This study examined the effects of fish protein in combination with fish oil on rat lipid metabolism. Male Wistar rats were divided into four groups and fed an AIN93G-based diet with casein (20%) + soybean oil (7%), casein (10%) + fish protein (10%) + soybean oil (7%), casein (20%) + soybean oil (5%) + fish oil (2%), and casein (10%) + fish protein (10%) + soybean oil (5%) + fish oil (2%) for 4 weeks. The dietary combination of fish protein and fish oil decreased the contents of serum triacylglycerol, serum cholesterol, liver triacylglycerol and liver cholesterol in addition to altering liver lipid fatty acid composition. These effects are partly due to the increase in fecal cholesterol, bile acid excretion, and increased enzyme activities of fatty acid β-oxidation in the liver. These data suggest that combined intake of fish protein and fish oil lead to both hypocholesterolemic and hypotriglyceridemic in serum and the liver, while sole intake of fish protein or fish oil decrease only cholesterol and triglyceride levels, respectively. These results suggest that combined intake of fish protein and fish oil may play beneficial roles in the prevention of lifestyle-related diseases as compared with sole fish protein intake.
本研究旨在探讨鱼蛋白与鱼油联合对大鼠脂代谢的影响。雄性 Wistar 大鼠被分为四组，分别喂食以 AIN93G 为基础的饲料，其中包含酪蛋白（20%）+大豆油（7%）、酪蛋白（10%）+鱼蛋白（10%）+大豆油（7%）、酪蛋白（20%）+大豆油（5%）+鱼油（2%）和酪蛋白（10%）+鱼蛋白（10%）+大豆油（5%）+鱼油（2%），为期 4 周。鱼蛋白和鱼油的联合饮食除了改变肝脏脂质脂肪酸组成外，还降低了血清三酰甘油、胆固醇、肝三酰甘油和肝胆固醇的含量。这些作用部分归因于粪便胆固醇、胆汁酸排泄增加，以及肝脏脂肪酸β-氧化酶活性的增加。这些数据表明，鱼蛋白和鱼油的联合摄入可导致血清和肝脏同时出现低胆固醇血症和低甘油三酯血症，而单独摄入鱼蛋白或鱼油则分别降低胆固醇和甘油三酯水平。这些结果表明，与单独摄入鱼蛋白相比，鱼蛋白和鱼油的联合摄入可能在预防与生活方式相关的疾病方面发挥有益作用。
