苏云金芽孢杆菌及其噬菌体在也门土壤中的存在情况。
Occurrence of Bacillus thuringiensis and their phages in Yemen soil.
作者信息
El-Didamony Gamal
机构信息
Department of Botany, Faculty of Science, Zagazig University, Zagazig, 44519 Egypt.
出版信息
Virusdisease. 2014 Jan;25(1):107-13. doi: 10.1007/s13337-013-0181-9. Epub 2013 Dec 1.
Bacillus thuringiensis (Bt) isolates were found in all samples of soil in nine Governorates of Yemen. From 384 isolates of Bacillus recovered from these soil samples after acetate selection, 104 isolates (27.1 %) were Bt. Five isolates of Bt were selected and insecticidal activity was tested against Culex pipiens, Callosobruchus maculatus and Spodoptera littoralis. The Bt isolate YH18 gave toxicity to all tested insects larvae. This study extended to isolate phages active against the selected Bt isolates. Five phages were isolated and classified into two groups of tailed phages. Four phages with long non-contractile tails and hexagonal heads (Siphoviridae) and one phage with very short tail and isometric head (Podoviridae). Susceptibility of selected Bt to infect by these phages was studied by spot-test technique. Also the Bt isolate no YH18 was resistant to all tested phages. This is the first report illustrates the diversity and the abundance of Bt and Bt phage in Yemen soil.
在也门九个省份的所有土壤样本中均发现了苏云金芽孢杆菌（Bt）菌株。从这些土壤样本经乙酸盐筛选后回收的384株芽孢杆菌分离物中，有104株（27.1%）为Bt。选取了5株Bt菌株，并对致倦库蚊、绿豆象和海滨夜蛾进行了杀虫活性测试。Bt分离株YH18对所有测试昆虫幼虫均具有毒性。本研究进一步分离了对所选Bt分离株具有活性的噬菌体。分离出5株噬菌体，并将其分为两组有尾噬菌体。4株噬菌体具有长的非收缩性尾和六边形头部（长尾噬菌体科），1株噬菌体具有非常短的尾和等轴状头部（短尾噬菌体科）。通过点滴试验技术研究了所选Bt对这些噬菌体感染的敏感性。此外，Bt分离株YH18对所有测试噬菌体均具有抗性。这是第一份说明也门土壤中Bt和Bt噬菌体的多样性和丰度的报告。
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