Guenther Leah E, Pyle Bryan W, Turgeon Thomas R, Bohm Eric R, Wyss Urs P, Schmidt Tannin A, Brandt Jan-M
Department of Mechanical and Manufacturing Engineering, University of Manitoba, Winnipeg, MB, Canada.
Proc Inst Mech Eng H. 2014 Feb;228(2):127-39. doi: 10.1177/0954411913517880. Epub 2014 Jan 15.
Biochemical analyses were performed on osteoarthritic and periprosthetic synovial fluid in order to propose changes to lubricant specifications currently outlined in orthopaedic wear testing standards. Osteoarthritic and periprosthetic synovial fluid samples were obtained from the hip and knee joints of 40 patients. The samples in each group were analysed and compared in order to identify differences between the protein concentration, constituent fractions, osmolality, thermal stability and the hyaluronic acid concentration and molecular weight distribution of osteoarthritic and periprosthetic synovial fluid. The average total protein concentration was approximately 30 g/L, which was much higher than the 20 g/L currently specified in the knee wear testing standard; however, the 30 g/L protein concentration matched the recently revised standard for hip simulator wear testing. No significant difference was found between the protein concentration, osmolality, thermal stability, and hyaluronic acid concentration of osteoarthritic and periprosthetic synovial fluid. The clinical data provided should be used to better define the composition of a more clinically relevant lubricant for orthopaedic wear testing.
对骨关节炎和假体周围滑液进行了生化分析，以便对目前骨科磨损测试标准中概述的润滑剂规格提出修改建议。从40名患者的髋关节和膝关节获取了骨关节炎和假体周围滑液样本。对每组样本进行分析和比较，以确定骨关节炎滑液和假体周围滑液在蛋白质浓度、组成成分、渗透压、热稳定性以及透明质酸浓度和分子量分布方面的差异。平均总蛋白浓度约为30 g/L，远高于目前膝关节磨损测试标准中规定的20 g/L；然而，30 g/L的蛋白浓度与最近修订的髋关节模拟器磨损测试标准相符。骨关节炎滑液和假体周围滑液在蛋白质浓度、渗透压、热稳定性和透明质酸浓度方面未发现显著差异。所提供的临床数据应用于更好地确定用于骨科磨损测试的更具临床相关性的润滑剂的成分。
