Hayashi K, Miller R G, Brownell K W
Anat Rec. 1987 Jul;218(3):275-83. doi: 10.1002/ar.1092180308.
A modified Golgi method combined with stereoscopy has been used to demonstrate the three-dimensional architecture of the sarcoplasmic reticulum (SR) and the T-system in human skeletal muscle. SR formed a continuous repeating network with a different structure dependent upon the sarcomere position. Intermyofibrillar SR contained three regions: 1) fenestrated collars overlying the M-band region, 2) terminal cisternae overlying the A-I region, and 3) a three-dimensional anastomosed tubular network overlying the Z-band region. Longitudinal and/or transverse SR tubules connected these regions. Subsarcolemmal SR was also composed of three regions: 1) transversely oriented polygonal meshes overlying the M-band, 2) single-layered tubules overlying the Z-band region, and 3) a loose network between the two. In the subsarcolemmal sarcoplasm, where mitochondria were aggregated, SR anastomosed loosely and showed nonfenestrated cisternae beneath the plasma membrane. The T-system was composed of transversely oriented networks overlying the A-I region with occasional longitudinal tubules connecting these networks.
一种改良的高尔基方法与立体显微镜相结合，已被用于展示人类骨骼肌中肌浆网（SR）和T系统的三维结构。SR形成了一个连续的重复网络，其结构因肌节位置而异。肌原纤维间的SR包含三个区域：1）覆盖M带区域的有孔环；2）覆盖A-I区域的终池；3）覆盖Z带区域的三维吻合管状网络。纵向和/或横向的SR小管连接这些区域。肌膜下SR也由三个区域组成：1）覆盖M带的横向多边形网孔；2）覆盖Z带区域的单层小管；3）两者之间的松散网络。在肌膜下肌浆中，线粒体聚集的地方，SR松散地吻合，在质膜下方显示无孔的池。T系统由覆盖A-I区域的横向网络组成，偶尔有纵向小管连接这些网络。
