Rambourg A, Segretain D
Anat Rec. 1980 May;197(1):33-48. doi: 10.1002/ar.1091970104.
The three-dimensional arrangement of mitochondria and endoplasmic reticulum in the red muscle fiber was studied both in thick sections of the rat diaphragm fixed in glutaraldehyde and impregnated with uranyl acetate followed by lead and copper citrate, and in thin sections of glutaraldehyde fixed tissue treated with ferrocyanide-reduced osmium. The mitochondria were located either at the periphery of the fiber, where they were spherical, or between the myofibrils, where they formed longitudinal columns of rectangular, slightly flattened elements. From both types of mitochondria, thin, elongated branches arose at right angles that formed transversely oriented mitochondrial pairs at the I band level. At the periphery of the fiber, the endoplasmic reticulum took the appearance of a subsarcolemmal network of tubular cisternae oriented parallel to the cell surface. In the juxtanuclear region, it was made up of spherical masses composed of tightly knitted tubules that were interconnected by more loosely anastomosed tubules. In between the myofibrils, it was composed of longitudinally oriented repetitive units whose structure varied according to their position in from of the A or I bands of the myofibrils. In front of the A band, the endoplasmic reticulum appeared as a single sheet of anastomotic tubules compressed between the adjacent myofibrils, whereas at the I band level, its tubular elements passed in front and behind the transverse expansions of the mitochondria to form an intricate ultilayered network in from of the Z line.
利用戊二醛固定、醋酸铀酰浸渍、柠檬酸铅和柠檬酸铜处理后的大鼠膈肌厚切片，以及亚铁氰化物还原锇处理的戊二醛固定组织薄切片，研究了红色肌纤维中线粒体和内质网的三维排列。线粒体要么位于纤维的周边，呈球形，要么位于肌原纤维之间，形成由矩形、略扁平的元件组成的纵向柱。这两种类型的线粒体都会伸出细长的分支，这些分支呈直角，在I带水平形成横向排列的线粒体对。在纤维周边，内质网呈现为平行于细胞表面的管状池的肌膜下网络。在近核区域，它由紧密编织的小管组成的球形团块构成，这些小管通过更松散吻合的小管相互连接。在肌原纤维之间，它由纵向排列的重复单元组成，其结构根据它们在肌原纤维A带或I带前方的位置而变化。在A带前方，内质网表现为夹在相邻肌原纤维之间的单层吻合小管，而在I带水平，其管状元件穿过线粒体横向扩张的前方和后方，在Z线前方形成一个复杂的多层网络。
