Arbeitsgruppe Biochemie der Morphogenese, Ruhr-Univesität Bochum, Postfach 2148, D-4630, Bochum, Federal Republic of Germany.
Planta. 1976 Jan;128(2):143-8. doi: 10.1007/BF00390315.
Fruiting body formation of Sordaria macrospora Auersw. is controlled by L-arginine and biotin when the fungus is grown on a synthetic nutrient medium containing optimal concentrations of fructose, KNO3, KH2PO4, MgSO4, and ZnSO4. Arginine and biotin operate in very low concentrations which exclude unspecific nutrient effects. In spite of the complicated interactions of arginine and biotin which are shown qualitatively (Figs. 3 and 4a) and quantitatively (Figs. 2 and 4b), the following conclusions are reached: 1. In the absence of biotin, the development of Sordaria macrospora is blocked at the stage of small protoperithecia. The external addition of biotin (optimal concentration: 3-12 μg/l) allows the formation of fertile fruiting bodies. This effect cannot be imitated by arginine. The biotin effect is discussed in connection with stimulated RNA synthesis.-2. The developmental velocity is influenced by the external addition of arginine. Without arginine but at permissible biotin concentrations, the total life cycle takes about 10 days, in the presence of arginine (1 mM), however, about 6 days.-3. The hyphal density, as well as the total number of fruiting bodies being produced, is controlled in a similar manner by biotin and arginine. The induction of fruiting body formation obviously takes place after the transgression of a critical hyphal density.
当真菌在含有最佳浓度果糖、KNO3、KH2PO4、MgSO4 和 ZnSO4 的合成营养培养基上生长时，Sordaria macrospora Auersw. 的生殖体形成受 L-精氨酸和生物素控制。精氨酸和生物素在非常低的浓度下起作用，排除了非特异性营养效应。尽管精氨酸和生物素的相互作用非常复杂（图 3 和 4a 定性显示，图 2 和 4b 定量显示），但得出以下结论：1. 在没有生物素的情况下，Sordaria macrospora 的发育在小原囊体阶段受阻。生物素的外部添加（最佳浓度：3-12μg/l）允许形成可育的生殖体。精氨酸不能模拟这种生物素效应。该生物素效应与刺激 RNA 合成有关。2. 发育速度受精氨酸的外部添加影响。在没有精氨酸但生物素浓度允许的情况下，总生命周期约为 10 天，而在存在精氨酸（1mM）的情况下，大约为 6 天。3. 菌丝密度以及产生的生殖体总数以类似的方式受生物素和精氨酸控制。生殖体形成的诱导显然发生在临界菌丝密度超过之后。
