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对. 的菌丝体和原基进行基因组调查和转录组分析。

Genome Survey and Transcriptome Analysis on Mycelia and Primordia of .

机构信息

Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, Fujian Province, China.

出版信息

Biomed Res Int. 2020 Jan 14;2020:1824183. doi: 10.1155/2020/1824183. eCollection 2020.

Abstract

, a type of edible straw-rotting mushroom with somewhat sweet taste and fragrance of almonds, has attracted considerable scientific and practical attention. High-throughput Illumina PE150 and PacBio RSII platform were employed to generate a genomic sequence. De novo assembly generated 36 contigs with 38,686,133 bp in size, containing 10,119 putative predicted genes. Additionally, we also studied transcriptional regulation of the mycelia and the primordia for exploration of genes involved in fruiting body formation. Expression profiling analysis revealed that 2,164 genes were upregulated in mycelia and 1,557 in primordia. Functional enrichment showed that differentially expressed genes associated with response to stress, ribosome biogenesis, arginine biosynthesis, and steroid biosynthesis pathway were more active in fruiting body. The genome and transcriptome analysis of provide valuable sequence resources and contribute to our understanding of genes related to the biosynthesis pathway of polysaccharide and benzaldehyde, as well as the fruiting body formation.

摘要

阿魏蘑,一种具有甜美口感和杏仁香味的可食用草腐菌,引起了相当多的科学和实践关注。我们采用高通量 Illumina PE150 和 PacBio RSII 平台生成了基因组序列。从头组装生成了 36 个 contigs,大小为 38686133bp,包含 10119 个推定的预测基因。此外,我们还研究了菌丝体和原基的转录调控,以探索参与子实体形成的基因。表达谱分析显示,在菌丝体中有 2164 个基因上调,在原基中有 1557 个基因上调。功能富集表明,与应激反应、核糖体生物发生、精氨酸生物合成和甾体生物合成途径相关的差异表达基因在子实体中更为活跃。对 的基因组和转录组分析为我们提供了有价值的序列资源,有助于我们理解与多糖和苯甲醛生物合成途径以及子实体形成相关的基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2901/6983287/101c1a5b072f/BMRI2020-1824183.001.jpg

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