Jonas R A, Schoen F J, Ziemer G, Britton L, Castaneda A R
Ann Thorac Surg. 1987 Sep;44(3):283-90. doi: 10.1016/s0003-4975(10)62075-9.
To assess the healing characteristics of knitted Dacron conduits sealed with fibrin glue, collagen cross-linked with glutaraldehyde, and collagen cross-linked with formaldehyde, composite conduits, each constructed from two of the three test materials, were implanted in the pulmonary and systemic circulations of sheep and dogs for 3 and 6 months. Relative rates and morphologies of sealant resorption and other histological findings were predicted by a previous study involving subcutaneous implantation of the same graft materials in the rat. In particular, both studies revealed that delayed resorption of collagen cross-linked with glutaraldehyde and collagen cross-linked with formaldehyde resulted in lack of adhesion between inner capsule and conduit, thereby causing focal hemorrhagic dissection in the circulatory implants. There were no important qualitative or quantitative differences in healing between sheep and dogs or in pulmonary versus systemic location of the conduit. Rendering an animal cyanotic by placing a pulmonary arterial-left atrial shunt or by increasing or decreasing the intraconduit systolic pressure did not obviously affect healing. The results emphasize that the biological consequences of sealed grafts are strongly dependent on the sealant material. Porosity control of knitted Dacron with biological sealants that undergo minimal or delayed resorption may lead to poor tissue adhesion and resultant complications.
为评估用纤维蛋白胶密封的针织涤纶导管、用戊二醛交联的胶原蛋白以及用甲醛交联的胶原蛋白的愈合特性，将由三种测试材料中的两种构建的复合导管植入绵羊和犬的肺循环和体循环中3个月和6个月。先前一项在大鼠皮下植入相同移植材料的研究预测了密封剂吸收的相对速率和形态以及其他组织学结果。特别是，两项研究均显示，用戊二醛交联的胶原蛋白和用甲醛交联的胶原蛋白的延迟吸收导致内囊与导管之间缺乏粘连，从而在循环植入物中引起局灶性出血性剥离。绵羊和犬之间的愈合情况或导管在肺循环与体循环中的位置在质量或数量上均无重要差异。通过放置肺动脉-左心房分流器或增加或降低导管内收缩压使动物发绀并未明显影响愈合。结果强调，密封移植物的生物学后果强烈依赖于密封剂材料。用吸收极少或延迟吸收的生物密封剂控制针织涤纶的孔隙率可能会导致组织粘连不良并引发并发症。
