Committee on Genetics and Department of Agronomy and Plant Genetics, Agricultural Experiment Station, University of Arizona, Tucson, Arizona, USA.
Theor Appl Genet. 1972 Jan;42(5):218-26. doi: 10.1007/BF00282510.
Polyacrylamide and starch gel electrophoresis were used to analyze the isozyme makeup of three enzyme systems (esterases, leucine aminopeptidases and catalases) from the dormant seeds of twenty-nine species within the genus Gossypium.Isozyme variation was observed for all three enzymes between the species of the different genome groups. The within species polymorphism noted for the esterases was not observed for the leucine aminopeptidase and catalase patterns. In general, only minor qualitative banding pattern differences distinguished the A and B genome species, whereas, band variations were greatest between the more distantly related species in the C, D and E genomes. Gossypium longicalyx (F genome) showed an overall banding pattern unique to itself. The species of the genomes (C, D, E and F) removed from the postulated area of genetic origin (Southern Africa) also exhibited greater isozyme variability than that of the wild species of the A and B genomes, both located in Southern Africa.Synthetic mixtures of seed extracts from parent species of recently formed synthetic allopolyploids produced additive isozyme patterns for esterase, leucine aminopeptidase and catalase that were closely comparable to the zymograms produced by their hybrids. In contrast all three enzyme systems showed significant qualitative isozyme variations between the three natural allotetraploids, G. tomentosum, G. barbadense and G. hirsutum when compared to the zymograms of the synthetic mixtures of their alleged parental forms.
聚丙烯酰胺和淀粉凝胶电泳用于分析休眠棉属 29 种种子中三种酶系统（酯酶、亮氨酸氨肽酶和过氧化氢酶）的同工酶组成。不同基因组组的物种之间观察到所有三种酶的同工酶变异。注意到酯酶的种内多态性在亮氨酸氨肽酶和过氧化氢酶模式中没有观察到。一般来说，仅细微的定性带型差异可以区分 A 和 B 基因组物种，而在 C、D 和 E 基因组中，亲缘关系较远的物种之间的带型变化最大。长绒棉（F 基因组）表现出独特的整体带型。远离假定遗传起源区（南非）的基因组（C、D、E 和 F）的物种的同工酶变异性也比位于南非的 A 和 B 基因组的野生物种更大。最近形成的合成异源多倍体的亲本物种的种子提取物的合成混合物产生了酯酶、亮氨酸氨肽酶和过氧化氢酶的加性同工酶模式，与它们的杂种产生的同工酶图谱非常相似。相比之下，当与假定亲本形式的合成混合物的同工酶图谱相比时，三个天然异源四倍体（G. tomentosum、G. barbadense 和 G. hirsutum）的三种酶系统都表现出显著的定性同工酶变异。
