荧光纳米显微镜：方法与应用
Fluorescence nanoscopy. Methods and applications.
作者信息
Requejo-Isidro Jose
机构信息
Unidad de Biofísica, CSIC-UPV/EHU, Barrio de Sarriena, s/n., 48940 Leioa, Spain.
出版信息
J Chem Biol. 2013 Jun 4;6(3):97-120. doi: 10.1007/s12154-013-0096-3.
Abstract
Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffraction-limited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them.
摘要
荧光纳米显微镜术是指用于荧光成像的实验技术和分析方法，其分辨率高于传统的、受衍射限制的显微镜。本综述解释了荧光纳米显微镜术背后的概念，并着重介绍了采集技术、获取高度详细的超分辨图像的标记策略以及从这些图像中提取有意义信息的定量方法方面的最新且有前景的进展。
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