Dipartimento di Scienze Ecologiche e Biologiche (DEB), Università degli Studi della Tuscia, Largo dell'Università, I-01100 Viterbo, Italy.
Dipartimento di Dipartimento di Scienze Agrarie, Ambientali ed Alimentari & Industrial Yeasts Collection DBVPG, Università di Perugia, Borgo XX Giugno 74, I-06121 Perugia, Italy.
Fungal Biol. 2014 Jan;118(1):61-71. doi: 10.1016/j.funbio.2013.11.002. Epub 2013 Nov 26.
Yeast strains isolated from rock samples collected from worldwide cold regions were identified by sequence analysis of the D1/D2 domains of the 26S rDNA gene and the ITS region followed by molecular phylogeny. Over 77% of yeasts isolates were Basidiomycota. Cryptococcus (orders Filobasidiales and Tremellales) and Rhodotorula (order Cystobasidiales) were the most frequent genera. About 40% of yeast isolates belonged to undescribed species. Almost all isolates were psychrotolerant. Urease and esterase were the most widely extracellular enzymatic activity at 4 °C and 20 °C. None of the strains exhibited extracellular protease, DNAse, cellulase, chitinase, and laccase activity. The taxonomic and ecological significance of yeasts associated to worldwide cold rocky habitats is discussed.
从全球寒冷地区采集的岩石样本中分离出的酵母菌株，通过对 26S rDNA 基因的 D1/D2 结构域和 ITS 区进行序列分析，并进行分子系统发育分析进行鉴定。超过 77%的酵母分离株属于担子菌门。隐球菌（Filobasidiales 和 Tremellales 目）和红酵母（Cystobasidiales 目）是最常见的属。约 40%的酵母分离株属于未描述的种。几乎所有的分离株都是耐冷的。脲酶和酯酶是在 4°C 和 20°C 下最广泛的胞外酶活性。没有菌株表现出胞外蛋白酶、DNA 酶、纤维素酶、几丁质酶和漆酶活性。讨论了与全球寒冷岩石栖息地相关的酵母的分类学和生态学意义。
