基于适配体的血小板衍生生长因子-BB（PDGF-BB）便携式生物传感器，可通过个人血糖仪读取。
Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout.
机构信息
MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
Department of Chemistry and Environmental Science, Minnan Normal University, Zhangzhou, Fujian 363000, China.
出版信息
Biosens Bioelectron. 2014 May 15;55:412-6. doi: 10.1016/j.bios.2013.12.041. Epub 2013 Dec 28.
A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0 × 10(-14) M~3.16 × 10(-12) M, and the detection limit is 2.9 fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples.
一种新型便携式生物传感器，基于适体与蛋白质之间的特异性识别，利用个人血糖仪（PGM）作为读出器，用于敏感和选择性检测血小板衍生生长因子-BB（PDGF-BB）。在 PDGF-BB 存在的情况下，与链霉亲和素磁珠（SA-PMPs）表面结合的 PDGF-BB 的主要适体与具有蔗糖酶功能的 PDGF-BB 的二级适体定量反应，形成稳定的复合物，导致蔗糖酶附着在 SA-PMPs 上。随后，蔗糖酶催化蔗糖水解生成大量葡萄糖，并通过 PGM 进行定量读出。PGM 的增强信号与 PDGF-BB 在 1.0×10(-14) M~3.16×10(-12) M 范围内的浓度呈线性关系，检测限为 2.9 fM。该便携式生物传感器已成功应用于唾液样品中 PDGF-BB 的测定。
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