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一种新型双核铱（III）配合物作为G-四链体选择性探针用于转录因子HIF-1α的荧光开启检测。

A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α.

作者信息

Lu Lihua, Wang Modi, Mao Zhifeng, Kang Tian-Shu, Chen Xiu-Ping, Lu Jin-Jian, Leung Chung-Hang, Ma Dik-Lung

机构信息

Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.

出版信息

Sci Rep. 2016 Mar 2;6:22458. doi: 10.1038/srep22458.

DOI:10.1038/srep22458
PMID:26932240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4773817/
Abstract

A novel dinuclear Ir(III) complex 5 was discovered to be specific to G-quadruplex DNA, and was utilized in a label-free G-quadruplex-based detection platform for transcription factor activity. The principle of this assay was demonstrated by using HIF-1α as a model protein. Moreover, this HIF-1α detection assay exhibited potential use for biological sample analysis.

摘要

一种新型双核铱（III）配合物5被发现对G-四链体DNA具有特异性，并被用于基于无标记G-四链体的转录因子活性检测平台。以缺氧诱导因子-1α（HIF-1α）作为模型蛋白证明了该检测方法的原理。此外，这种HIF-1α检测方法在生物样品分析中具有潜在应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/860de8c886e7/srep22458-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/defc369d7145/srep22458-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/961c349257a1/srep22458-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/a1398e090d78/srep22458-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/4fcb0234ba40/srep22458-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/860de8c886e7/srep22458-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/defc369d7145/srep22458-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/961c349257a1/srep22458-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/a1398e090d78/srep22458-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/4fcb0234ba40/srep22458-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c7/4773817/860de8c886e7/srep22458-f5.jpg

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