Chaufan Gabriela, Coalova Isis, Ríos de Molina María Del Carmen
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 2 Pabellón, 4 piso, Ciudad Autónoma de Buenos Aires, CP 1428, Argentina. Email:
Int J Toxicol. 2014 Jan-Feb;33(1):29-38. doi: 10.1177/1091581813517906. Epub 2014 Jan 16.
In the present study, the effects on oxidative balance and cellular end points of glyphosate, aminomethylphosphonic acid (AMPA), and a glyphosate formulation (G formulation) were examined in HepG2 cell line, at dilution levels far below agricultural recommendations. Our results show that G formulation had toxic effects while no effects were found with acid glyphosate and AMPA treatments. Glyphosate formulation exposure produced an increase in reactive oxygen species, nitrotyrosine formation, superoxide dismutase activity, and glutathione (GSH) levels, while no effects were observed for catalase and GSH-S-transferase activities. Also, G formulation triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. Aminomethylphosphonic acid exposure produced an increase in GSH levels while no differences were observed in other antioxidant parameters. No effects were observed when the cells were exposed to acid glyphosate. These results confirm that G formulations have adjuvants working together with the active ingredient and causing toxic effects that are not seen with acid glyphosate.
在本研究中，在远低于农业推荐浓度的稀释水平下，研究了草甘膦、氨甲基膦酸（AMPA）和一种草甘膦制剂（G制剂）对HepG2细胞系氧化平衡和细胞终点的影响。我们的结果表明，G制剂具有毒性作用，而酸性草甘膦和AMPA处理未发现有影响。草甘膦制剂暴露导致活性氧增加、硝基酪氨酸形成、超氧化物歧化酶活性和谷胱甘肽（GSH）水平升高，而过氧化氢酶和GSH - S - 转移酶活性未观察到影响。此外，G制剂触发了半胱天冬酶3/7的激活，从而在该细胞系中诱导了凋亡途径。氨甲基膦酸暴露使GSH水平升高，而其他抗氧化参数未观察到差异。当细胞暴露于酸性草甘膦时未观察到影响。这些结果证实，G制剂中的佐剂与活性成分共同作用，产生了酸性草甘膦未见的毒性作用。
