Coalova Isis, Ríos de Molina María Del Carmen, Chaufan Gabriela
Departamento de Química Biológica, IQUIBICEN - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Departamento de Química Biológica, IQUIBICEN - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Toxicol In Vitro. 2014 Oct;28(7):1306-11. doi: 10.1016/j.tiv.2014.06.014. Epub 2014 Jul 3.
In the present study, the influence of the spray adjuvant on the toxicity effects of a glyphosate formulation was examined in HEp-2 cell line. We determined the median lethal concentration (LC50) of Atanor® (glyphosate formulation), Impacto® (spray adjuvant) and the mixture of both agrochemicals. We also compared the toxicities of the pesticides individually and in mixture and we analyzed the effects on oxidative balance from each treatment. Our results showed that all the agrochemicals assayed induce dose and time-dependent cytotoxicity and that the toxicity of Impacto® with Atanor® (mixture) was additive on HEp-2 cell line. All the agrochemicals assayed produced an increase in catalase activity and glutathione levels, while no effects were observed for superoxide dismutase and glutathione-S-transferase activities. We found an important increase in ROS production in cells treated with Atanor® and mixture. Besides, all the agrochemicals used triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. In conclusion, our results demonstrated that the addition of adjuvant to glyphosate formulation increase the toxicity of the mixture in cell culture. Furthermore, cell culture exposed to agrochemical mixture showed an increased ROS production and antioxidant defenses.
在本研究中，在人喉表皮样癌细胞系（HEp-2细胞系）中检测了喷雾助剂对草甘膦制剂毒性作用的影响。我们测定了Atanor®（草甘膦制剂）、Impacto®（喷雾助剂）以及这两种农用化学品混合物的半数致死浓度（LC50）。我们还比较了这些农药单独使用和混合使用时的毒性，并分析了每种处理对氧化平衡的影响。我们的结果表明，所有检测的农用化学品均诱导剂量和时间依赖性细胞毒性，并且Impacto®与Atanor®（混合物）对HEp-2细胞系的毒性具有相加作用。所有检测的农用化学品均使过氧化氢酶活性和谷胱甘肽水平升高，而超氧化物歧化酶和谷胱甘肽-S-转移酶活性未观察到影响。我们发现用Atanor®和混合物处理的细胞中活性氧（ROS）生成显著增加。此外，所有使用的农用化学品均引发半胱天冬酶3/7激活，从而在该细胞系中诱导凋亡途径。总之，我们的结果表明，向草甘膦制剂中添加助剂会增加混合物在细胞培养中的毒性。此外，暴露于农用化学品混合物的细胞培养物显示ROS生成增加和抗氧化防御增强。
