Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, 2801 South University Avenue, Little Rock, AR 72204, USA. The Oncology Institute, Prof. Dr. I. Chiricuta, Republicii, No. 34-36, RO-400015, Cluj-Napoca, Romania.
Nanotechnology. 2014 Feb 14;25(6):065102. doi: 10.1088/0957-4484/25/6/065102. Epub 2014 Jan 16.
Multidimensional scaffolds are considered to be ideal candidates for regenerative medicine and tissue engineering based on their potential to provide an excellent microenvironment and direct the fate of the cultured cells. More recently, the use of stem cells in medicine has opened a new technological opportunity for controlled tissue formation. However, the mechanism through which the substrate directs the differentiation of stem cells is still rather unclear. Data concerning its specific surface chemistry, topology, and its signaling ability need to be further understood and analyzed. In our study, atomic force microscopy was used to study the stiffness, roughness, and topology of the collagen (Coll) and metallized collagen (MC) substrates, proposed as an excellent substrate for regenerative medicine. The importance of signaling molecules was studied by constructing a new hybrid signaling substrate that contains both collagen and laminin extracellular matrix (ECM) proteins. The cellular response-such as attachment capability, proliferation and cardiac and neuronal phenotype expression on the metallized and non-metallized hybrid substrates (collagen + laminin)-was studied using MTT viability assay and immunohistochemistry studies. Our findings indicate that such hybrid materials could play an important role in the regeneration of complex tissues.
多维支架被认为是再生医学和组织工程的理想候选材料，因为它们有可能提供一个极好的微环境，并直接影响培养细胞的命运。最近，干细胞在医学中的应用为控制组织形成开辟了新的技术机会。然而，基底物质如何指导干细胞的分化机制仍相当不清楚。关于其特定表面化学、拓扑结构和信号传递能力的数据需要进一步理解和分析。在我们的研究中，原子力显微镜被用于研究胶原（Coll）和金属化胶原（MC）基质的硬度、粗糙度和拓扑结构，这些基质被提议作为再生医学的优秀基底物质。通过构建一种新的包含胶原和层粘连蛋白细胞外基质（ECM）蛋白的杂交信号基底物质，研究了信号分子的重要性。使用 MTT 细胞活力测定和免疫组织化学研究，研究了细胞在金属化和非金属化杂交基底物质（胶原+层粘连蛋白）上的附着能力、增殖和心脏及神经元表型表达等细胞反应。我们的研究结果表明，这种杂交材料可能在复杂组织的再生中发挥重要作用。
