National Institute for Materials Science, Biomaterials Center, Tsukuba, Ibaraki, Japan.
Expert Opin Biol Ther. 2010 Dec;10(12):1717-28. doi: 10.1517/14712598.2010.534079.
Biomimetic scaffolds and substrates of extracellular matrices (ECMs) play an important role in the regulation of cell function and in the guidance of new tissue regeneration, as an ECM has the intrinsic cues necessary to communicate with and dictate to cells.
This paper reviews the latest developments in ECM scaffolds and substrates obtained from decellularized tissues, organs or cultured cells and their application in tissue engineering. The ECM composition, structure, interaction with surrounding cells, preparation method and usage in the regeneration of various tissues and organs are summarised.
The advantages and challenges of decellularized matrices are highlighted.
Similarity in the composition, microstructure and biomechanical properties of the decellularized scaffolds and substrates to those of the native tissues and organs maximizes the promotion effect in the regeneration of both structural and functional tissues and organs. Simple tissues as well as complicated organs have been decellularized and decellularization methods have been optimized to completely remove the cellular components while keeping the ECM intact.
仿生支架和细胞外基质 (ECM) 的基质在调节细胞功能和引导新组织再生方面发挥着重要作用，因为 ECM 具有与细胞进行交流和控制细胞所必需的内在线索。
本文综述了从去细胞化组织、器官或培养细胞中获得的 ECM 支架和基质的最新进展及其在组织工程中的应用。总结了 ECM 的组成、结构、与周围细胞的相互作用、制备方法以及在各种组织和器官再生中的应用。
强调了去细胞化基质的优点和挑战。
去细胞化支架和基质在组成、微观结构和生物力学特性上与天然组织和器官相似，最大限度地提高了对结构和功能组织和器官再生的促进作用。简单的组织和复杂的器官都已经被去细胞化，并且优化了去细胞化方法，以在保持 ECM 完整的同时，完全去除细胞成分。
