Department of Botany and Microbiology, University of Oklahoma, 73069, Norman, Oklahoma, USA.
Planta. 1975 Jan;124(1):77-87. doi: 10.1007/BF00390070.
The change in protein content and composition of the cotyledons of Pisum sativum L. cv. Burpeeana during germination was studied. Protein depletion from the cotyledons was slow during the first 4 days of germination but became rapid on the 5th day and by the 16th day the majority of the protein had disappeared. During the first 4 days the depletion of the globulins exceeded that of the albumins; legumin appeared to be degraded slightly more rapidly than vicilin during the early phase of germination. Sodium-dodecylsulfate (SDS) electrophoresis of SDS- and dithiothreitol-dissociated globulins indicated that before rapid protein depletion there were marked changes in the component composition of the major globulins legumin and vicilin. The onset of rapid protein depletion was associated with an increase in the level of an acid-sulfhydryl protease in the cotyledons. These findings indicate that the reserve globulins undergo modifications prior to their eventual hydrolysis.
研究了豌豆 cv. Burpeeana 子叶在萌发过程中蛋白质含量和组成的变化。在萌发的前 4 天，子叶中的蛋白质耗竭速度较慢，但在第 5 天迅速增加，到第 16 天，大部分蛋白质已经消失。在最初的 4 天里，球蛋白的耗竭速度超过了白蛋白；在萌发的早期阶段，豌豆球蛋白似乎比菜豆球蛋白降解得稍微快一些。用十二烷基硫酸钠（SDS）和二硫苏糖醇（DTT）解离球蛋白的 SDS 电泳表明，在快速蛋白质耗竭之前，主要球蛋白豌豆球蛋白和菜豆球蛋白的成分组成发生了明显变化。快速蛋白质耗竭的开始与子叶中酸性巯基蛋白酶水平的增加有关。这些发现表明，储备球蛋白在最终水解之前会发生修饰。
