Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823.
Plant Physiol. 1973 Apr;51(4):708-13. doi: 10.1104/pp.51.4.708.
Protease activity increased in attached cotyledons of germinated peas (Pisum sativum L. cv. Alaska) as the stored proteins declined but did not increase in excised cotyledons incubated for the same length of time. Cotyledons of seeds germinated in the presence of a casein hydrolysate solution developed less protease activity than did those germinated on water. These results suggest that accumulation of amino acids regulates the protease level in the cotyledons of germinating peas.In contrast to protease, alpha- and beta-amylase increased during incubation of excised pea cotyledons. Their increase was inhibited by abscisic acid. Abscisic acid did not inhibit (14)C-leucine incorporation into protein or reduce the respiratory rate in the cotyledons; hence, its effect on amylase formation was not the result of a general inhibition of metabolism. An ether-soluble acid fraction, which would contain any abscisic acid present in the material, inhibited amylase formation more when it was obtained from imbibed seeds than when it was obtained from cotyledons of seeds germinated for 10 days. These and other results suggest that amylase formation in germinating peas is regulated by abscisic acid.
在萌发的豌豆（Pisum sativum L. cv. Alaska）的附着子叶中，蛋白酶活性随着储存蛋白的减少而增加，但在相同时间内培养的离体子叶中并没有增加。在存在酪蛋白水解物溶液的情况下萌发的种子的子叶比在水中萌发的子叶产生的蛋白酶活性低。这些结果表明，氨基酸的积累调节了萌发豌豆子叶中的蛋白酶水平。与蛋白酶相反，α-和β-淀粉酶在离体豌豆子叶的孵育过程中增加。脱落酸抑制了它们的增加。脱落酸不抑制（14）C-亮氨酸掺入蛋白质或降低子叶中的呼吸速率；因此，它对淀粉酶形成的影响不是对代谢的普遍抑制的结果。一种乙醚可溶的酸级分，如果材料中存在任何脱落酸，那么从吸胀的种子中获得的比从萌发 10 天的种子的子叶中获得的更能抑制淀粉酶的形成。这些和其他结果表明，脱落酸调节萌发豌豆中的淀粉酶形成。
