Department of Botany and Microbiology, University College of Wales, SY23 3DA, Aberystwyth, UK.
Planta. 1975 Jan;124(3):319-28. doi: 10.1007/BF00388695.
Homogenates of etiolated wheat leaves contain increased levels of acidic gibberellin (GA)-like substances following treatment with red light. Differential centrifugation of homogenates indicates that the response is confined to the 1000 g (or plastid) fraction. Irradiation of suspensions of intact etioplasts also increases the level of extractable GA-like activity. Phytochrome can be detected spectrophotometrically in preparations of etioplasts. The response in etioplasts can be inhibited by chloramphenicol, but not by cycloheximide, and partially by Amo 1618. The GA-like substances produced in etioplasts seem capable of passing into the surrounding medium within 20 min.
黄化小麦叶片的匀浆在红光处理后，含有更多的酸性赤霉素（GA）样物质。匀浆的差速离心表明，这种反应局限于 1000g（或质体）部分。完整质体悬浮液的辐照也会增加可提取的 GA 样活性水平。在质体制剂中可以分光光度法检测到光敏色素。质体中的反应可以被氯霉素抑制，但不能被环己酰亚胺抑制，并且部分被 Amo 1618 抑制。质体中产生的 GA 样物质似乎能够在 20 分钟内进入周围介质。
