Laboratory of Genetics, University of Nijmegen, The Netherlands.
Theor Appl Genet. 1969 Jan;39(7):330-4. doi: 10.1007/BF00281915.
The mutagenic activity of various combinations of formaldehyde, formic acid, acetic acid and hydrochloric acid was investigated by a sex-linked lethal test. All combinations were mutagenic and showed a mutation pattern from which it is concluded that in feeding experiments spermatocytes I are especially sensitive to the pairs of chemicals tested. In vapour experiments all germ cell stages were found to be susceptible.The presence of volatile acids was found to be necessary for the mutagenic activity of formaldehyde in the vapour state. Mutagenic effects were also observed in larvel feeding experiments, in which only these acids were added to the medium. Experiments with stabilized pH at 7.5 did not show a significant mutagenic effect of formaldehyde.It is postulated that the tested agents are catalase inhibitors, which promote the formation of peroxides or free radicals which interfere with DNA replication, thus producing mutations.
采用伴性连锁致死试验研究了甲醛、甲酸、乙酸和盐酸的各种组合的诱变活性。所有组合均具有诱变活性，并表现出一种突变模式，可以得出结论，在饲喂实验中，精母细胞 I 对所测试的化学物质对特别敏感。在蒸气实验中，发现所有生殖细胞阶段都易感。发现挥发性酸的存在对于甲醛在蒸气状态下的诱变活性是必要的。在幼虫饲喂实验中也观察到了诱变效应，其中仅向培养基中添加这些酸。在稳定 pH 值为 7.5 的实验中，甲醛没有表现出显著的诱变效应。据推测，所测试的试剂是过氧化氢酶抑制剂，它促进过氧化物或自由基的形成，这些物质会干扰 DNA 复制，从而产生突变。
