AFRC Photosynthesis Research Group, Department of Pure and Applied Biology, Imperial College of Science and Technology, SW7 2BB, London, UK.
Photosynth Res. 1987 Jan;13(2):185-98. doi: 10.1007/BF00035240.
Nearest neighbour relationships within the LHC2-PS2 complex were investigated by using the reversible crosslinking agent dithiobis(succinimidyl propionate) (DSP). This was accomplished by treating PS2-enriched membranes, prepared from chloroplasts of Pisum sativum, with the crosslinker followed by diagonal electrophoresis of the solubilised polypeptides.Analysis of the off-diagonal spot patterns produced by crosslinker cleavage and second dimension electrophoresis was made on the basis of: staining with Coomassie blue or silver, labelling with [(35)S]-methionine, and sensitivity to 1 M NaCl washing. It was concluded that LHC2 polypeptides crosslinked with several components of the PS2 complex and that the extrinsic polypeptides associated with water oxidation, having approximate molecular weights of 16 and 23 kDa, crosslink to form homodimers. The latter finding suggests that there may be more than one copy of each of these polypeptides per PS2 complex.
通过使用可逆交联剂二硫代双（琥珀酰亚胺基丙酸酯）（DSP），研究了 LHC2-PS2 复合物内的最近邻关系。这是通过用交联剂处理从豌豆叶绿体中制备的富含 PS2 的膜来完成的，然后对可溶性多肽进行对角线电泳。根据以下方法分析交联剂切割和二维电泳产生的非对角斑点模式：考马斯亮蓝或银染色、[(35)S]-蛋氨酸标记和对 1 M NaCl 洗涤的敏感性。得出的结论是，LHC2 多肽与 PS2 复合物的几个成分交联，并且与水氧化相关的外在多肽，分子量约为 16 和 23 kDa，交联形成同源二聚体。后一种发现表明，每个 PS2 复合物中可能有多个这些多肽的副本。
