豌豆叶绿体 DNA 编码膜多肽 D2 的基因的定位和核苷酸序列。
Localization and nucleotide sequence of the gene for the membrane polypeptide D2 from pea chloroplast DNA.
机构信息
Department of Genetics, The Royal Veterinary and Agricultural University, Bülowsvej 13, 61-1, 1., 1870, Copenhagen V, Denmark.
出版信息
Plant Mol Biol. 1984 Jul;3(4):191-9. doi: 10.1007/BF00029654.
The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleotides upstream from the initiation codon and there are two sets of putative promotor sequences in the 5' flanking region. The polypeptide has a high content of hydrophobic amino acids, predominatly grouped in clusters of 20 or more residues. The 3' end of the D2 gene is overlapped by 50 nucleotides of a second open reading frame (UORF I) which is at least 369 nucleotides long. Based on current data we suggest the D2 polypeptide to be a constituent of photosystem II (PSII).
D2 膜多肽的基因已被定位在豌豆（Pisum sativum）叶绿体基因组上。本文呈现了该基因及其侧翼区域的核苷酸序列。序列中唯一的大开放阅读框编码一个 MW 为 39.5 kD 的蛋白质。在起始密码子上游 6 个核苷酸处有一个潜在的核糖体结合位点，在 5'侧翼区有两组假定的启动子序列。该多肽具有高比例的疏水性氨基酸，主要聚集在 20 个或更多残基的簇中。D2 基因的 3'端被第二个开放阅读框（UORF I）的 50 个核苷酸重叠，该阅读框至少长 369 个核苷酸。根据目前的数据，我们推测 D2 多肽是光系统 II（PSII）的组成部分。
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