Department of Biology, Princeton University, 08540, Princeton, New Jersey, USA.
Planta. 1975 Jan;122(1):1-9. doi: 10.1007/BF00385399.
Crown-gall teratoma tissues of tobacco (Nicotiana tabacum L.) grow in culture at 25° in the absence of added auxin or cell-division factors. The capacity of these tissues to grow without added auxin is temperature sensitive. At 35° auxin provided as α-naphthalene acetic acid or 2,4-dichlorophenoxyacetic acid is required for continued exponential growth. Moreover at 35° the auxin dose response of teratoma tissues shifts from the pattern characteristic of autonomous tumor tissues at 25° to the pattern characteristic of auxin-requiring normal tissues. Incubation of the tissues at 35° also appears to induce lasting changes in teratoma phenotype. Using relative growth of tissues with and without added auxin as a criterion, pretreatment with auxin induces a stable increase in auxin-autotrophy, while pretreatment without auxin induces a stable decrease in auxin-autotrophy. These results are consistent with a positive feedback mechanism for stable changes in auxin-autotrophy.
烟草（Nicotiana tabacum L.）的冠瘿瘤组织在 25℃下的培养中无需添加生长素或细胞分裂因子即可生长。这些组织在没有添加生长素的情况下生长的能力对温度敏感。在 35℃下，需要提供α-萘乙酸或 2,4-二氯苯氧乙酸作为生长素才能继续指数生长。此外，在 35℃下，畸胎瘤组织的生长素剂量反应从 25℃下自主肿瘤组织的特征模式转变为需要生长素的正常组织的特征模式。在 35℃下孵育组织似乎也会诱导畸胎瘤表型的持久变化。使用有和没有添加生长素的组织的相对生长作为标准，生长素预处理诱导生长素自养的稳定增加，而没有生长素的预处理诱导生长素自养的稳定减少。这些结果与生长素自养稳定变化的正反馈机制一致。
