Department of Agronomy, University of Illinois, 61801, Urbana, IL, USA.
Planta. 1977 Jan;134(2):103-8. doi: 10.1007/BF00384957.
Auxin autotrophy was studied in cultured carrot (Daucus carota L.), tobacco (Nicotiana tabacum L.), and potato (Solanum tuberosum L.) cell lines. Of 10 carrot lines resistant to 5-methyltryptophan (5MT), which accumulate free tryptophan (trp) because of an altered control enzyme, 5 were auxinautotrophic while the normal parent line was not. Carrot lines selected from the same parent line as resistant to other amino-acid analogs were not auxinautotrophic, like the parent. The only 5MT-resistant potato line studied was also auxin-autotrophic while the normal line was only partially auxin-autotrophic. The tobacco lines which accumulated free trp were not auxin-autotrophic, and no auxin-autotrophic tobacco lines were selected by screening for growth in medium lacking 2,4-dichlorophenoxyacetic acid (2,4-D). Several auxin-autotrophic carrot and potato lines were selected from the normal lines and none of these lines were resistant to 5MT. Length of time in culture and difficulty in selecting auxin-autotrophic lines were correlated on the 3 normal carrot lines studied. The addition of trp or indole to the culture medium would partially alleviate the auxin requirement of the normal lines studied. 2,4-D (0.4 mg/l) stimulated the growth of all auxin-autotrophic carrot lines.
在培养的胡萝卜（Daucus carota L.）、烟草（Nicotiana tabacum L.）和马铃薯（Solanum tuberosum L.）细胞系中研究了生长素自养。在 10 种对 5-甲基色氨酸（5MT）具有抗性的胡萝卜系中，由于控制酶发生改变而积累游离色氨酸（trp），其中 5 种为生长素自养型，而正常亲本系则不是。从与对其他氨基酸类似物具有抗性的相同亲本系中选择的胡萝卜系也不是生长素自养型，与亲本系一样。研究的唯一一种对 5MT 具有抗性的马铃薯系也是生长素自养型，而正常系仅部分是生长素自养型。积累游离 trp 的烟草系不是生长素自养型，并且通过筛选缺乏 2,4-二氯苯氧基乙酸（2,4-D）的培养基中的生长来选择生长素自养型烟草系是不可能的。从正常系中选择了几种生长素自养型的胡萝卜和马铃薯系，而这些系中没有一种对 5MT 具有抗性。在所研究的 3 种正常胡萝卜系中，培养时间的长短和选择生长素自养系的难度相关。在培养基中添加 trp 或吲哚可以部分缓解所研究的正常系对生长素的需求。2,4-D（0.4 mg/l）刺激所有生长素自养型胡萝卜系的生长。
