Department of Biology, Indiana University, Bloomington, Indiana 47405.
Plant Physiol. 1982 Feb;69(2):389-92. doi: 10.1104/pp.69.2.389.
The endogenous levels of auxin and cytokinin in teratoma and unorganized tobacco (Nicotiana tabacum L. var Wisconsin #38) crown gall tumor tissues were determined. Teratoma tissues contain levels of auxin and cytokinin favorable for shoot formation, whereas unorganized tumors contain levels of auxin that suppress shoot formation. This conclusion is based upon the observation that when levels of auxin and cytokinin similar to those found in a teratoma were added to the growth medium of nontumorous tobacco tissue, shoot formation resulted; when levels similar to those found in unorganized tumors were added, the normal tissue grew as unorganized callus.
测定了畸胎瘤和无组织烟草(Nicotiana tabacum L. var Wisconsin #38)冠瘿瘤组织中内源生长素和细胞分裂素的水平。畸胎瘤组织中生长素和细胞分裂素的水平有利于芽的形成,而无组织肿瘤中生长素的水平抑制芽的形成。这一结论是基于这样的观察,即当向非肿瘤烟草组织的生长培养基中添加类似于畸胎瘤中发现的生长素和细胞分裂素水平时,会导致芽的形成;而当添加类似于无组织肿瘤中发现的水平时,正常组织则生长为无组织的愈伤组织。