甜橙愈伤组织抗氨基喋呤缺陷型中次黄嘌呤磷酸核糖转移酶的缺乏。

A deficiency of hypoxanthine phosphoribosyltransferase in a sycamore callus resistant to azaguanine.

机构信息

Department of Biochemistry, University of Cambridge, CB2 1 QW, Cambridge, UK.

出版信息

Planta. 1975 Jan;123(1):79-89. doi: 10.1007/BF00388062.

Abstract

Sycamore (Acer pseudoplatanus L.) calluses resistant to azaguanine were isolated after mutagenesis with nitrosoguanidine. One callus SNC61 was highly variable in its growth on 10 μg/ml azaguanine and it was concluded that its resistance was not due to a stable alteration in the callus. Another callus SNE31 was isolated after 4.5 months culture in 0.5 or 1 μg/ml azaguanine which had a 10-fold increase in resistance to azaguanine. This was shown to be accompanied by a reduction to 50% of the normal level of hypoxanthine phosphoribosyltransferase (E.C.2.4.2.8).

摘要

经过亚硝胍诱变，成功分离出抗氮鸟嘌呤的梧桐（Acer pseudoplatanus L.）愈伤组织。一个愈伤组织 SNC61 在 10μg/ml 氮鸟嘌呤上的生长变化很大，因此推断其抗性不是由于愈伤组织的稳定改变。另一个愈伤组织 SNE31 在 0.5 或 1μg/ml 氮鸟嘌呤中培养 4.5 个月后被分离出来，其对氮鸟嘌呤的抗性增加了 10 倍。这伴随着次黄嘌呤磷酸核糖基转移酶（E.C.2.4.2.8）正常水平的降低至 50%。