完整大鼠附睾脂肪细胞内乙酰辅酶A羧化酶磷酸化的证明
Demonstration of the phosphorylation of acetyl-coenzyme A carboxylase within intact rat epididymal fat-cells.
作者信息
Brownsey R W, Hughes W A, Denton R M
出版信息
Biochem J. 1977 Dec 15;168(3):441-5. doi: 10.1042/bj1680441.
Abstract
Intact rat epididymal fat-cells were incubated with 32Pi and the intracellular proteins separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. One of the phosphorylated proteins has the same RF value as [14C]biotin-labelled acetyl-CoA carboxylase purified from fat-cells and is specifically precipitated after incubation with antiserum raised against acetyl-CoA carboxylase. No significant changes in the extent of phosphorylation of acetyl-CoA carboxylase were detected after exposure of the cells to insulin.
摘要
将完整的大鼠附睾脂肪细胞与32Pi一起孵育，然后通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分离细胞内蛋白质。其中一种磷酸化蛋白的Rf值与从脂肪细胞中纯化的[14C]生物素标记的乙酰辅酶A羧化酶相同，并且在用抗乙酰辅酶A羧化酶产生的抗血清孵育后会被特异性沉淀。在细胞暴露于胰岛素后，未检测到乙酰辅酶A羧化酶磷酸化程度的显著变化。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Acetyl coenzyme A carboxylase from rat liver. Purification and demonstration of different subunits.大鼠肝脏乙酰辅酶A羧化酶。不同亚基的纯化与鉴定
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A new method of preparation of troponin I (inhibitory protein) using affinity chromatography. Evidence for three different forms of troponin I in striated muscle.一种利用亲和层析法制备肌钙蛋白I（抑制蛋白）的新方法。横纹肌中存在三种不同形式肌钙蛋白I的证据。
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Differential effects of metabolites on the active and inactive forms of hepatic acetyl CoA carboxylase.代谢物对肝脏乙酰辅酶A羧化酶活性形式和非活性形式的不同影响。
Arch Biochem Biophys. 1974 Oct;164(2):490-501. doi: 10.1016/0003-9861(74)90059-9.
5
Regulation of hepatic acetyl coenzyme A carboxylase by phosphorylation and dephosphorylation.通过磷酸化和去磷酸化对肝脏乙酰辅酶A羧化酶的调节。
Arch Biochem Biophys. 1974 Oct;164(2):478-89. doi: 10.1016/0003-9861(74)90058-7.
6
Hormonal regulation of adipose-tissue acetyl-Coenzyme A carboxylase by changes in the polymeric state of the enzyme. The role of long-chain fatty acyl-Coenzyme A thioesters and citrate.通过改变酶的聚合状态对脂肪组织乙酰辅酶A羧化酶进行激素调节。长链脂肪酰辅酶A硫酯和柠檬酸的作用。
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7
Isolation of acetyl CoA apocarboxylase by sepharose-avidin affinity chromatography.通过琼脂糖-抗生物素蛋白亲和色谱法分离乙酰辅酶A羧化酶原
Anal Biochem. 1973 Nov;56(1):191-5. doi: 10.1016/0003-2697(73)90183-8.
8
Insulin and the regulation of adipose tissue acetyl-coenzyme A carboxylase.胰岛素与脂肪组织乙酰辅酶A羧化酶的调节
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Presence of two polypeptide chains comprising fatty acid synthetase.存在由脂肪酸合成酶组成的两条多肽链。
Proc Natl Acad Sci U S A. 1975 May;72(5):1940-4. doi: 10.1073/pnas.72.5.1940.
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