Awad Faez, Baylis Matthew, Jones Richard C, Ganapathy Kannan
a Institute of Infection and Global Health , University of Liverpool , Neston , UK.
Avian Pathol. 2014;43(2):125-9. doi: 10.1080/03079457.2014.885114. Epub 2014 Feb 12.
The feasibility of using Flinders Technology Associates (FTA) cards for the molecular detection of avian metapneumovirus (aMPV) by reverse transcriptase-polymerase chain reaction (RT-PCR) was investigated. Findings showed that no virus isolation was possible from aMPV-inoculated FTA cards, confirming viral inactivation upon contact with the cards. The detection limits of aMPV from the FTA card and tracheal organ culture medium were 10(1.5) median ciliostatic doses/ml and 10(0.75) median ciliostatic doses/ml respectively. It was possible to perform molecular characterization of both subtypes A and B aMPV using inoculated FTA cards stored for up to 60 days at 4 to 6°C. Tissues of the turbinate, trachea and lung of aMPV-infected chicks sampled either by direct impression smears or by inoculation of the tissue homogenate supernatants onto the FTA cards were positive by RT-PCR. However, the latter yielded more detections. FTA cards are suitable for collecting and transporting aMPV-positive samples, providing a reliable and hazard-free source of RNA for molecular characterization.
研究了使用弗林德斯技术协会（FTA）卡通过逆转录聚合酶链反应（RT-PCR）对禽偏肺病毒（aMPV）进行分子检测的可行性。研究结果表明，从接种了aMPV的FTA卡中无法分离出病毒，证实病毒与卡片接触后会失活。FTA卡和气管器官培养基中aMPV的检测限分别为10（1.5）半数纤毛制动剂量/毫升和10（0.75）半数纤毛制动剂量/毫升。使用在4至6°C下储存长达60天的接种FTA卡，可以对aMPV的A和B亚型进行分子特征分析。通过直接印片或通过将组织匀浆上清液接种到FTA卡上采集的aMPV感染雏鸡的鼻甲、气管和肺组织，经RT-PCR检测呈阳性。然而，后者检测到的更多。FTA卡适用于收集和运输aMPV阳性样本，为分子特征分析提供可靠且无危害的RNA来源。
