Mukai Kiyotaka, Kuda Yuhichi, Shibamoto Toshishige, Tanida Mamoru, Kurata Yasutaka, Yokoyama Hitoshi
Department of Nephrology, Kanazawa Medical University, Ishikawa, 920-0293, Japan.
Department of Physiology II, Kanazawa Medical University, Ishikawa, 920-0293, Japan.
J Physiol Sci. 2018 Sep;68(5):689-697. doi: 10.1007/s12576-017-0583-3. Epub 2017 Dec 5.
We determined the renal responses to anaphylaxis and the effects of a nitric oxide synthesis inhibitor, L-NAME, in anesthetized rats and isolated perfused rat kidneys. After the ovalbumin antigen injection, the sensitized rats showed transient and substantial decreases in mean blood pressure and renal blood flow and an increase in renal vascular resistance. Creatinine clearance, a measure of renal function, decreased to 53% baseline at 2 h after antigen. L-NAME pretreatment significantly enhanced the antigen-induced renal vasoconstriction and renal dysfunction. Moreover, plasma creatinine levels significantly increased only in the L-NAME pretreated rats. Separately, in isolated perfused kidneys, we observed the antigen-induced renal vasoconstriction and its augmentation by L-NAME. In conclusion, the renal vascular response to the antigen is vasoconstriction, which is enhanced by L-NAME in both isolated perfused rat kidneys and anesthetized rats; it is accompanied by renal dysfunction, which is also augmented by L-NAME.
我们在麻醉大鼠和离体灌注大鼠肾脏中，确定了过敏反应的肾脏反应以及一氧化氮合成抑制剂L - NAME的作用。卵清蛋白抗原注射后，致敏大鼠的平均血压和肾血流量出现短暂且显著的下降，肾血管阻力增加。作为肾功能指标的肌酐清除率在抗原注射后2小时降至基线的53%。L - NAME预处理显著增强了抗原诱导的肾血管收缩和肾功能障碍。此外，仅在L - NAME预处理的大鼠中，血浆肌酐水平显著升高。另外，在离体灌注肾脏中，我们观察到抗原诱导的肾血管收缩以及L - NAME对其的增强作用。总之，肾脏对抗原的血管反应是血管收缩，在离体灌注大鼠肾脏和麻醉大鼠中L - NAME均可增强这种反应；它还伴有肾功能障碍，L - NAME也会加剧这种障碍。
