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[耳软骨的抗原性及活体保存的影响。2:耳软骨抗原的鉴定:大鼠动物实验研究]

[Antigenicity of the auricular cartilage and the influence of vital preservation. 2: Identification of antigens of the auricular cartilage: animal experiment studies in rats].

作者信息

Jakse R

机构信息

Univ. HNO-Klinik Graz.

出版信息

Laryngol Rhinol Otol (Stuttg). 1987 Jul;66(7):362-5.

PMID:2443779
Abstract

We could demonstrate the existence of class I and class II antigens on the chondrocytes of the auricular cartilage in inbred strain rats (Louvain/M/Him, Fischer 344). Indirect fluorescence and related techniques were used. The cartilage was stored in Ham's nutrient medium F 12 or in the tissue culture for 8-10 days before allotransplantation. A complete disappearance of the antigens could not be achieved by means of the vital conservation. Grafting did not modify further the distribution of the antigens; in 2 experiments only renewed strong coloration of the chondrocyte-membranes and the surrounding matrix could be observed.

摘要

我们能够在近交系大鼠(鲁汶/M/希姆、费希尔344)耳廓软骨的软骨细胞上证实I类和II类抗原的存在。采用了间接荧光及相关技术。在同种异体移植前,软骨保存在哈姆氏营养培养基F12或组织培养中8 - 10天。通过活体保存无法使抗原完全消失。移植并未进一步改变抗原的分布;仅在2个实验中观察到软骨细胞膜和周围基质再次出现强烈染色。

相似文献

1
[Antigenicity of the auricular cartilage and the influence of vital preservation. 2: Identification of antigens of the auricular cartilage: animal experiment studies in rats].[耳软骨的抗原性及活体保存的影响。2:耳软骨抗原的鉴定:大鼠动物实验研究]
Laryngol Rhinol Otol (Stuttg). 1987 Jul;66(7):362-5.
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[Antigenicity of the auricular cartilage and its modification by vital preservation. 1. Xenogeneic transplantation of human auricular concha cartilage to the rabbit].[耳软骨的抗原性及其通过活体保存的改变。1. 人耳甲腔软骨异种移植至兔]
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[Effect of glutaraldehyde on the species-specific antigenicity of xenogeneic cartilage].[戊二醛对异种软骨种属特异性抗原性的影响]
Arch Sci Med (Torino). 1982 Oct-Dec;139(4):455-8.
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Class II antigenicity of human cartilage: relevance to the use of homologous cartilage graft for reconstructive surgery.人类软骨的II类抗原性:与同种异体软骨移植用于重建手术的相关性。
Ann Plast Surg. 1991 Jun;26(6):541-3. doi: 10.1097/00000637-199106000-00007.
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[A method for culturing cartilage].[一种培养软骨的方法]
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Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage.单层培养和工程化弹性软骨中儿科耳廓软骨细胞的基因表达分析
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引用本文的文献

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The effect of gamma-interferon on HLA class II antigen expression on isolated human nasal chondrocytes.γ干扰素对分离的人鼻软骨细胞上HLA II类抗原表达的影响。
Eur Arch Otorhinolaryngol. 1990;247(5):287-90. doi: 10.1007/BF00176539.