Jakse R
Univ. HNO-Klinik Graz.
Laryngol Rhinol Otol (Stuttg). 1987 Jul;66(7):362-5.
We could demonstrate the existence of class I and class II antigens on the chondrocytes of the auricular cartilage in inbred strain rats (Louvain/M/Him, Fischer 344). Indirect fluorescence and related techniques were used. The cartilage was stored in Ham's nutrient medium F 12 or in the tissue culture for 8-10 days before allotransplantation. A complete disappearance of the antigens could not be achieved by means of the vital conservation. Grafting did not modify further the distribution of the antigens; in 2 experiments only renewed strong coloration of the chondrocyte-membranes and the surrounding matrix could be observed.
我们能够在近交系大鼠(鲁汶/M/希姆、费希尔344)耳廓软骨的软骨细胞上证实I类和II类抗原的存在。采用了间接荧光及相关技术。在同种异体移植前,软骨保存在哈姆氏营养培养基F12或组织培养中8 - 10天。通过活体保存无法使抗原完全消失。移植并未进一步改变抗原的分布;仅在2个实验中观察到软骨细胞膜和周围基质再次出现强烈染色。