Bujía J, Wilmes E, Hammer C, Kastenbauer E
Department of Otolaryngology, Ludwig Maximilians University, Munich, Germany.
Ann Plast Surg. 1991 Jun;26(6):541-3. doi: 10.1097/00000637-199106000-00007.
The presence and distribution of class II transplantation antigens was studied on fresh and Merthiolate-preserved human nasal, tracheal, auricular, and rib cartilage using monoclonal antibodies in an indirect immunoperoxidase method. Substantial class II antigen expression was found on cells of the superficial area of the perichondrium of the nasal, auricle, and tracheal cartilages. In contrast, cartilage tissue lacked cells with detectable class II antigens. Our results indicate that the host response to fresh cartilage graft is induced by class II antigens presented in the perichondrium. A complete disappearance of this class II antigenicity of perichondrium can be achieved by means of an adequate Merthiolate preservation.
采用间接免疫过氧化物酶法，使用单克隆抗体研究了新鲜的和硫柳汞保存的人鼻、气管、耳廓及肋软骨上II类移植抗原的存在及分布情况。在鼻、耳廓和气管软骨的软骨膜表层区域的细胞上发现了大量II类抗原表达。相比之下，软骨组织缺乏可检测到II类抗原的细胞。我们的结果表明，宿主对新鲜软骨移植物的反应是由软骨膜中呈现的II类抗原诱导的。通过适当的硫柳汞保存可以使软骨膜的这种II类抗原性完全消失。
