Hou Li-Xia, Ying She, Yang Xiao-Wen, Yu Zhang, Li Hui-Min, Qin Xin-Min
a Guangxi Key Laboratory of Rare and Endangered Animal Ecology , College of Life Science, Guangxi Normal University , Guilin , Guangxi , PR China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):102-3. doi: 10.3109/19401736.2013.873923. Epub 2014 Jan 17.
The complete mitochondrial genome sequence of Papilio bianor was determined in the present paper. The complete mtDNA from P. bianor was 15,358 base pairs in length and contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and a control region. The P. bianor genes were in the same order and orientation as the completely sequenced mitogenomes of other lepidopteran species. To determine the phylogentic position of P. bianor with related species within Papilionidae, the Bayesian phylogenetic tree was reconstructed with the concatenated nucleotide dataset of the 13 protein-coding genes. The phylogenetic trees confirmed that P. bianor and four species of Papilionidae clustered into a clade, and shared a close relationship with Papilio maraho. Meanwhile, the molecular phylogenetic trees also confirmed that Papilionidae is a monophyletic group, and Pieridae is closely related with Lycaenidae and Nymphalidae.
本文测定了碧凤蝶的完整线粒体基因组序列。碧凤蝶的完整线粒体DNA长度为15358个碱基对,包含13个蛋白质编码基因(PCGs)、2个rRNA基因、22个tRNA基因和一个控制区。碧凤蝶的基因排列顺序和方向与其他已完全测序的鳞翅目物种的有丝分裂基因组相同。为了确定碧凤蝶在凤蝶科内与相关物种的系统发育位置,利用13个蛋白质编码基因的串联核苷酸数据集重建了贝叶斯系统发育树。系统发育树证实,碧凤蝶和凤蝶科的四个物种聚为一个分支,与台湾凤蝶关系密切。同时,分子系统发育树也证实凤蝶科是一个单系类群,粉蝶科与灰蝶科和蛱蝶科关系密切。