Qin Feng, Jiang Guo-Fang, Zhou Shan-Yi
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, P.R. China.
Mitochondrial DNA. 2012 Apr;23(2):123-5. doi: 10.3109/19401736.2011.653805. Epub 2012 Mar 13.
In this study, we sequenced the complete mitochondrial genome of Teinopalpus aureus guangxiensis (Lepidoptera: Papilionidae), which is considered as an endemic species in China. It is listed as a vulnerable species by International Union for Conservation of Nature and Natural Resources Red List and also a first class endangered species in China. The complete mtDNA from T. aureus guangxiensis was 15,235 base pairs in length and contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region. The T. aureus guangxiensis genes were in the same order and orientation as the completely sequenced mitogenomes of other lepidopteran species. All PCGs of T. aureus guangxiensis mitogenome start with a typical ATN codon and terminate in the common stop codon TAA, except that ND1 gene uses TTA, ND3 gene uses ATT, and ND4 and ND4L gene use TAA. The phylogenetic relationships were reconstructed with the concatenated sequences of the 13 PCGs of the mitochondrial genome, and phylogenetic results confirmed that Nymphalidae, Lycaenidae, Papilionidae, Pieridae are monophyletic clades.
在本研究中，我们对金斑喙凤蝶广西亚种（鳞翅目：凤蝶科）的完整线粒体基因组进行了测序，该亚种被认为是中国的特有物种。它被国际自然保护联盟红色名录列为易危物种，在中国也是一级濒危物种。金斑喙凤蝶广西亚种的完整线粒体DNA长度为15,235个碱基对，包含13个蛋白质编码基因（PCGs）、2个rRNA基因、22个tRNA基因和一个控制区。金斑喙凤蝶广西亚种的基因顺序和方向与其他已完全测序的鳞翅目物种的线粒体基因组相同。金斑喙凤蝶广西亚种线粒体基因组的所有PCGs均以典型的ATN密码子起始，并以常见的终止密码子TAA终止，但ND1基因使用TTA，ND3基因使用ATT，ND4和ND4L基因使用TAA。利用线粒体基因组13个PCGs的串联序列重建了系统发育关系，系统发育结果证实蛱蝶科、灰蝶科、凤蝶科、粉蝶科均为单系类群。
