Yang Ling, Wei Zhao-Jun, Hong Gui-Yun, Jiang Shao-Tong, Wen Long-Ping
School of Life Sciences, University of Science and Technology of China, Hefei, 230027, People's Republic of China.
Mol Biol Rep. 2009 Jul;36(6):1441-9. doi: 10.1007/s11033-008-9334-0. Epub 2008 Aug 12.
Using long-polymerase chain reaction (Long-PCR) method, we determined the complete nucleotide sequence of the mitochondrial genome (mitogenome) of Phthonandria atrilineata. The complete mtDNA from P. atrilineata was 15,499 base pairs in length and contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region. The P. atrilineata genes were in the same order and orientation as the completely sequenced mitogenomes of other lepidopteran species. The nucleotide composition of P. atrilineata mitogenome was biased toward A + T nucleotides (81.02%), and the 13 PCGs show different A + T contents that range from 73.25% (cox1) to 92.12% (atp8). Phthonandria had the canonical set of 22 tRNA genes, that fold in the typical cloverleaf structure described for metazoan mt tRNAs, with the unique exception of trnS(AGN). The phylogenetic relationships were reconstructed with the concatenated sequences of the 13 PCGs of the mitochondrial genome, which confirmed that P. atrilineata is most closely related to the superfamily Bombycoidea.
我们采用长聚合酶链反应（Long-PCR）方法，测定了黑线小卷蛾（Phthonandria atrilineata）线粒体基因组（线粒体基因组）的完整核苷酸序列。黑线小卷蛾的完整线粒体DNA长度为15499个碱基对，包含13个蛋白质编码基因（PCGs）、2个rRNA基因、22个tRNA基因和一个控制区。黑线小卷蛾的基因顺序和方向与其他鳞翅目物种完全测序的线粒体基因组相同。黑线小卷蛾线粒体基因组的核苷酸组成偏向于A+T核苷酸（81.02%），13个PCGs显示出不同的A+T含量，范围从73.25%（cox1）到92.12%（atp8）。黑线小卷蛾有一套典型的22个tRNA基因，它们折叠成后生动物线粒体tRNA所描述的典型三叶草结构，唯一的例外是trnS(AGN)。利用线粒体基因组13个PCGs的串联序列重建了系统发育关系，证实黑线小卷蛾与蚕蛾总科关系最为密切。
