一种核糖核酸酶的模型底物。

Model substrates for an RNA enzyme.

作者信息

McClain W H, Guerrier-Takada C, Altman S

机构信息

Department of Bacteriology, University of Wisconsin, Madison 53706.

出版信息

Science. 1987 Oct 23;238(4826):527-30. doi: 10.1126/science.2443980.

DOI:10.1126/science.2443980

PMID:2443980

Abstract

M1 RNA, the catalytic RNA subunit of Escherichia coli ribonuclease P, can cleave novel transfer RNA (tRNA) precursors that lack specific domains of the normal tRNA sequence. The smallest tRNA precursor that was cleaved efficiently retained only the domain of the amino acid acceptor stem and the T stem and loop. The importance of the 3' terminal CCA nucleotide residues in the processing of both novel and normal tRNA precursors implies that the same enzymatic function of M1 RNA is involved.

摘要

M1 RNA是大肠杆菌核糖核酸酶P的催化RNA亚基，它能够切割新型转运RNA（tRNA）前体，这些前体缺乏正常tRNA序列的特定结构域。能被有效切割的最小tRNA前体仅保留了氨基酸接受茎以及T茎环结构域。3'末端CCA核苷酸残基在新型和正常tRNA前体加工过程中的重要性表明，M1 RNA发挥了相同的酶促功能。