蒙古沙鼠体内苯并[a]芘的代谢：开蓬和灭蚁灵诱导的影响

Benzo[a]pyrene metabolism in the Mongolian gerbil: influence of chlordecone and mirex induction.

Crouch L S, Ebel R E

Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061.

Xenobiotica. 1987 Jul;17(7):859-67. doi: 10.3109/00498258709043995.

Abstract

The metabolism of benzo[a]pyrene (BP) by gerbil hepatic microsomes is increased following induction by phenobarbital (PB), chlordecone, mirex and 3-methylcholanthrene (3-MC). 2. By several criteria including the influence of alpha-naphthoflavone (alpha-NF) on BP-hydroxylase activity and BP-metabolite profiles, the cytochromes P-450 responsible for benzo[a]pyrene metabolism appear to be similar in microsomes isolated from PB-, chlordecone-, or mirex-treated gerbils. The cytochromes P-450 present in microsomes isolated from control animals and those treated with 3-MC are different from each other and from those present in PB, chlordecone, or mirex microsomes by the same criteria. 3. Of the inducers used, only PB induced microsomal epoxide hydrolase activity.

摘要

经苯巴比妥（PB）、十氯酮、灭蚁灵和3-甲基胆蒽（3-MC）诱导后，沙鼠肝微粒体对苯并[a]芘（BP）的代谢增加。2. 根据包括α-萘黄酮（α-NF）对BP-羟化酶活性和BP代谢产物谱的影响等多个标准，从经PB、十氯酮或灭蚁灵处理的沙鼠中分离出的微粒体中，负责苯并[a]芘代谢的细胞色素P-450似乎相似。根据相同标准，从对照动物和经3-MC处理的动物中分离出的微粒体中存在的细胞色素P-450彼此不同，且与PB、十氯酮或灭蚁灵微粒体中存在的细胞色素P-450也不同。3. 在所用的诱导剂中，只有PB诱导了微粒体环氧化物水解酶活性。