Little P J, James M O, Pritchard J B, Bend J R
J Environ Pathol Toxicol Oncol. 1984 Jul;5(4-5):309-20.
Benzo(a)pyrene hydroxylase (AHH) activities were measured in hepatic microsomes from untreated and 3-methylcholanthrene (3-MC) treated southern flounder (Paralichthys lethostigma). The AHH activities of only three of the feral animals appeared to be uninduced, i.e., AHH activities were very low (14 to 22 pmol/min/mg protein) and were stimulated approximately 2-fold by 7,8-benzoflavone (7,8-BF)-in vitro. All remaining untreated animals had higher AHH activities (72 to 623 pmol/min/mg) which were markedly inhibited by 7,8-BF (78.2 +/- 3.0%). These fish were much more similar to the 3-MC induced fish (843 +/- 120 pmol/min/mg and 81% inhibition by 7,8-BF) than to the uninduced animals. However, all three groups (feral with low AHH activity, feral with high AHH activity and 3-MC treated) showed very similar patterns of benzo(a)pyrene (BP) metabolism with a marked preference for benzo-ring oxidation (60% of total metabolites). The pattern of BP metabolism in flounder is in marked contrast to that of the control rat, but is similar to that of the 3-MC induced rat. These results suggest that, at least in the pattern of BP metabolism, flounder hepatic microsomes and their constitutive cytochromes P-450 from untreated and polycyclic hydrocarbon induced fish are similar to rat hepatic cytochromes P-450 induced by polycyclic aromatic hydrocarbon administration.
测定了未经处理和经3-甲基胆蒽（3-MC）处理的南方鲆（Paralichthys lethostigma）肝脏微粒体中的苯并(a)芘羟化酶（AHH）活性。仅3只野生南方鲆的AHH活性似乎未被诱导，即AHH活性非常低（14至22 pmol/分钟/毫克蛋白质），且在体外被7,8-苯并黄酮（7,8-BF）刺激约2倍。其余所有未经处理的南方鲆具有较高的AHH活性（72至623 pmol/分钟/毫克），这些活性被7,8-BF显著抑制（78.2±3.0%）。与未诱导的南方鲆相比，这些南方鲆与经3-MC诱导的南方鲆（843±120 pmol/分钟/毫克，被7,8-BF抑制81%）更为相似。然而，所有三组（AHH活性低的野生南方鲆、AHH活性高的野生南方鲆和经3-MC处理的南方鲆）显示出非常相似的苯并(a)芘（BP）代谢模式，对苯环氧化具有明显偏好（占总代谢产物的60%）。南方鲆的BP代谢模式与对照大鼠的模式形成显著对比，但与经3-MC诱导的大鼠的模式相似。这些结果表明，至少在BP代谢模式方面，未经处理和多环烃诱导的鱼类的南方鲆肝脏微粒体及其组成型细胞色素P-450与经多环芳烃给药诱导的大鼠肝脏细胞色素P-450相似。
