Roelcke D, Meiser R J, Brücher H
Blut. 1979 Sep;39(3):217-24. doi: 10.1007/BF01008451.
Two examples of human IgM cold agglutinins agglutinated human RBC only after enzyme treatment in vitro. Proteases were optimally effective, neuraminidase was also effective. The cold agglutinins did not coat native RBC but were directed against "cryptic" RBC determinants. The cold agglutinins belonged to the anti -I/-i complex indicating a "new" type of I/i determinants. They were strongly accessible to cold agglutinin interaction on native RBC of a patient with congenital dyserythropoietic anaemia. Enzyme treatment of RBC was shown to be not only suited for defining cold agglutinin specificities but also essential for detecting the "new" type of cold agglutinins, obviously causing autoimmune haemolytic anaemia in vivo.
两例人类IgM冷凝集素仅在体外酶处理后才凝集人类红细胞。蛋白酶效果最佳，神经氨酸酶也有效。这些冷凝集素不包被天然红细胞，而是针对“隐蔽”的红细胞决定簇。这些冷凝集素属于抗-I/-i复合物，表明存在一种“新型”的I/i决定簇。它们在先天性红细胞生成异常性贫血患者的天然红细胞上与冷凝集素的相互作用很强。红细胞的酶处理不仅适用于确定冷凝集素的特异性，而且对于检测“新型”冷凝集素至关重要，显然这种冷凝集素在体内会导致自身免疫性溶血性贫血。
