Krstić M K, Djurković D
Department of Pharmacology, Faculty of Medicine, Belgrade, Yugoslavia.
Arch Int Physiol Biochim. 1987 Jun;95(2):153-8. doi: 10.3109/13813458709104528.
In rats the effect of inhibition of the brain cholinesterase activity on the pressor and heart rate responses to 5-hydroxytryptamine (5-HT), administered into the lateral cerebral ventricle (l.c.v.) was examined. After administration of physostigmine (twice in a small dose of 2.5 micrograms l.c.v., 20 and 15 min before the second injection of 5-HT), the pressor effect of 5-HT (5 micrograms) was strongly reduced or almost abolished, its pure tachycardia was reduced or reversed into a bradycardia and its pure bradycardia was diminished or reversed into a tachycardia. The type of the cardiovascular response to ACh (5 micrograms l.c.v., 20 min after the second administration of 5-HT) indicates that the modification of the cardiovascular response to 5-HT was accompanied by inhibition of the brain cholinesterase activity. Thus, it seems that a functionally competent cholinesterase in the brain is necessary for the generation of the 5-HT-induced pressor response. The present experiments provide further evidence that there is a cholinergic link in the pathway by which serotonergic mechanisms in the preoptic-anterior hypothalamic area rise blood pressure and support the idea that the same link exists in the pathway(s) mediating the heart rate responses to intracerebroventricular administration of 5-HT.
在大鼠中，研究了抑制脑胆碱酯酶活性对向侧脑室注射5-羟色胺（5-HT）后升压和心率反应的影响。在给予毒扁豆碱后（分两次小剂量2.5微克向侧脑室注射，在第二次注射5-HT前20分钟和15分钟各注射一次），5-HT（5微克）的升压作用大幅降低或几乎消失，其单纯的心动过速减弱或转变为心动过缓，而单纯的心动过缓则减弱或转变为心动过速。对乙酰胆碱（5微克向侧脑室注射，在第二次注射5-HT后20分钟）的心血管反应类型表明，对5-HT心血管反应的改变伴随着脑胆碱酯酶活性的抑制。因此，似乎脑内功能正常的胆碱酯酶对于5-HT诱导的升压反应的产生是必要的。本实验进一步证明，视前区-下丘脑前区的血清素能机制升高血压的途径中存在胆碱能联系，并支持这样的观点，即在介导对脑室内注射5-HT的心率反应的途径中也存在相同的联系。
